Disease Markers

Disease Markers / 2009 / Article

Open Access

Volume 27 |Article ID 849784 | 7 pages | https://doi.org/10.3233/DMA-2009-0653

A Homogeneous HLA-B*27 Genotyping Assay Using Dried Reagent Mixtures

Received28 Oct 2009
Accepted28 Oct 2009

Abstract

The presence of HLA-B*27 allele with patients suspected with ankylosing spondylitis can be used in the diagnostic process. We have developed an assay for typing for the HLA-B*27 in whole blood dried on sample collection cards using pre-dried reagent wells and homogeneous time-resolved fluorescence based PCR approach. Essentially only the sample needs to be added to the dry ready-to-use reaction well in order to start the homogenous amplification assay. The method was validated with 229 samples also typed with an existing DELFIA-based method and results of both assays were 100% concordant. The dried reagents were shown to be stable at least up to eight weeks at room temperature without any decline in their performance.

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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