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Disease Markers
Volume 28, Issue 5, Pages 323-331
http://dx.doi.org/10.3233/DMA-2010-0712

Analysis of Polymorphisms in Genes (AGT, MTHFR, GPIIIa, and GSTP1) Associated with Hypertension, Thrombophilia and Oxidative Stress in Mestizo and Amerindian Populations of México

Rocio Juárez-Velázquez,1,2 Patricia Canto,3 Thelma Canto-Cetina,4 Hector Rangel-Villalobos,5 Haydee Rosas-Vargas,1 Maricela Rodríguez,6 Samuel Canizales-Quinteros,7 Ana Claudia Velázquez Wong,1 Rosa María Ordoñez-Razo,1 Guadalupe Vilchis-Dorantes,8 and Ramón Mauricio Coral-Vázquez9,10

1Unidad de Investigación Médica en Genética Humana, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México, D.F., Mexico
2Present adress: Laboratorio de Cultivo de Tejidos, Departamento de Investigación en Genética Humana, Instituto Nacional de Pediatría, México, D.F., Mexico
3División de Investigación Biomédica, Subdirección de Enseñanza e Investigación, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico
4Laboratorio de Biología de la Reproducción, Departamento de Salud Reproductiva y Genética, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Mérida Yucatán, Mexico
5Laboratorio de Genética Molecular (CUCiénega-U de G), Departamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega, Universidad de Guadalajara, Ocotlán, Jalisco, Mexico
6Unidad de Investigación Médica en Nutrición. Centro Médico Nacional Siglo XXI, Coordinación de Investigación en Salud, Instituto Mexicano del Seguro Social, México, D.F., Mexico
7Unidad de Biología Molecular y Medicina Genómica, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", México, D.F., Mexico
8Laboratorio de Genética Forense, Dirección de la Coordinación General de Servicios Periciales, Procuraduría General de la Republica (PGR), Mexico City, Mexico
9Laboratorio Multidisciplinario, Sección de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional, México, DF., Mexico
10División de Medicina Genómica, Subdirección de Enseñanza e Investigación, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico

Received 25 June 2010; Accepted 25 June 2010

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Several polymorphisms related to hypertension, thrombophilia, and oxidative stress has been associated with the development of cardiovascular disease. We analyzed the frequency of M235T angiotensinogen (AGT), A222V 5,10 methylenete-trahydrofolate reductase (MTHFR), L33P glycoprotein IIIa (GPIIIa), and I105V glutathione S-transferase P1 (GSTP1) polymorphisms in 285 individuals belonging to Mexican-Mestizo and five Amerindian population from México, by real time PCR allelic discrimination. Allele and genotype frequencies were compared using χ2 tests.

All populations followed the Hardy Weinberg equilibrium for assay markers with the exception of the Triki, whose were in Hardy Weinberg dysequilibrium for the glutathione S-transferase P1 polymorphism.

Interestingly, according to all the analyzed single nucleotide polymorphisms (SNPs), the Triki population was the most differentiated and homogeneous group of the six populations analyzed. A comparison of our data with those previously published for some Caucasian, Asian and Black populations showed quite significant differences. These differences were remarkable with all the Mexican populations having a lower frequency of the 105V allele of the glutathione S-transferase P1 and reduced occurrence of the 222A allele of the 5,10 methylenetetrahydrofolate reductase. Our results show the genetic diversity among different Mexican populations and with other racial groups.