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Disease Markers
Volume 31, Issue 6, Pages 379-386

Isolation and Identification of Concanavalin A Binding Glycoproteins from Human Seminal Plasma: A Step Towards Identification of Male Infertility Marker Proteins

Anil Kumar Tomar,1 Balwinder Singh Sooch,2 Isha Raj,3 Sarman Singh,4 Tej P. Singh,1 and Savita Yadav1

1Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India
2Department of Biotechnology, Punjabi University, Patiala, India
3School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
4Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India

Received 15 December 2011; Accepted 15 December 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Human seminal plasma contains a large array of proteins of clinical importance which are essentially needed to maintain the reproductive physiology of spermatozoa and for successful fertilization. Thus, isolation and identification of seminal plasma proteins is of paramount significance for their biophysical characterization and functional analysis in reproductive physiological processes. In this study, we have isolated Concanavalin-A binding glycoproteins from human seminal plasma and subsequently identified them by MALDI-TOF/MS analysis. The major proteins, as identified in this study, are Aminopeptidase N, lactoferrin, prostatic acid phosphatase, zinc-alpha-2-glycoprotein, prostate specific antigen, progestagen-associated endometrial protein, Izumo sperm-egg fusion protein and prolactin inducible protein. This paper also reports preliminary studies to identify altered expression of these proteins in oligospermia and azoospermia in comparison to normospermia. In oligospermia, five proteins were found to be downregulated while in azoospermia, four proteins were downregulated and two proteins were upregulated. Thus, this study is of immense biomedical interest towards identification of potential male infertility marker proteins in seminal plasma.