Disease Markers

Disease Markers / 2012 / Article

Open Access

Volume 32 |Article ID 582107 | https://doi.org/10.3233/DMA-2011-0875

Beatrice Mohelnikova-Duchonova, Martin Oliverius, Eva Honsova, Pavel Soucek, "Evaluation of Reference Genes and Normalization Strategy for Quantitative Real-Time PCR in Human Pancreatic Carcinoma", Disease Markers, vol. 32, Article ID 582107, 8 pages, 2012. https://doi.org/10.3233/DMA-2011-0875

Evaluation of Reference Genes and Normalization Strategy for Quantitative Real-Time PCR in Human Pancreatic Carcinoma

Received23 Feb 2012
Accepted23 Feb 2012

Abstract

Histologically verified pairs (n = 10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw Cq values correlated with the degree of RNA degradation. This correlation was abolished by normalization to Cq of 18S endogenous control gene. Both geNorm and NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the same most stable genes. We have thus identified suitable reference genes for future expression studies in pancreatic carcinoma. Normalization method reducing the effects of RNA degradation on the quality of results was also developed.

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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