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Disease Markers
Volume 33, Issue 1, Pages 35-42
http://dx.doi.org/10.3233/DMA-2012-0901

Relation between MicroRNA Expression in Peritoneal Dialysis Effluent and Peritoneal Transport Characteristics

Jin Chen,1,2 Philip Kam-Tao,1 Bonnie Ching-Ha Kwan,1 Kai-Ming Chow,1 Ka-Bik Lai,1 Cathy Choi-Wan Luk,1 and Cheuk-Chun Szeto1

1Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China
2Division of Nephrology, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu, Sichuan, China

Received 5 April 2012; Accepted 5 April 2012

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background: The role of microRNAs (miRNAs) in peritoneal transport is uncertain.

Methods: We studied 82 new peritoneal dialysis (PD) patients, 22 prevalent patients without ultrafiltration problem, and 6 patients with documented ultrafiltration problem. Peritoneal transport was determined by standard peritoneal equilibration test (PET). RNA was extracted from the PD effluent after PET, and intra-peritoneal expression of miRNA targets were quantified.

Results: There were significant difference in the PDE expressions of miR-15a and miR-21. There were modest inverse correlations between ultrafiltration volume and PDE expression of miR-17 (r = −0.198, p = 0.041) and miR-377 (r = −0.201, p = 0.041). There was an inverse correlations between dialysate-to-plasma creatinine concentration at 4 hours and PDE expression of miR-192 (r = −0.199, p = 0.040); while mass transfer area coefficient of creatinine correlated with PDE expression of miR-192 (r = −0.191, p = 0.049) and miR-377 (r = 0.201, p = 0.041). Amongst 7 randomly selected patients who had repeat PET after one year, there was a significant correlation between baseline PDE expression of miR-377 and change in ultrafiltration volume (r = −0.852, p = 0.015).

Conclusion: The miRNA expression in PDE, including miR-15a, miR-17, miR-21, miR-30, miR-192, and miR-377, correlated with peritoneal transport characteristics. Our result suggests that miRNA may play a role in the regulation of peritoneal membrane function.