Association of TAP Gene Polymorphisms and Risk of Cervical Intraepithelial Neoplasia

Table 1

Analysis of the five TAP gene polymorphisms.

SNP	Allele specific probes	PCR product	Sequencing primer	Annealing temperature
TAP1 1341	biotin-5′-TACCTGGTGCGAGGCCTATGTC	150 bp	5′-AAAAGCAGAGGCAGG	64°C
	5′-AGCGGGCCAACTCCATGAAC
TAP1 2254	5′-CATGTGGCTATACCGTTC	135 bp	5′-TTGCTCTGCAGAGGTAG-3'	52°C
	biotin-5′-GGATAAGTACACACGGTTTC
TAP2 1135	5′-GAAGTCTGTCGCTATAAAGAG	124 bp	5′-CGCCTTGTACCTGCT-3′	52°C
	biotin-5′-GGCTCCTTTCACAACCAC
TAP2 1693	biotin-5′-GCACAGGTGGTTTCAGTTGG	96 bp	5′-GCTCTGCAGCCCATA-3′	58°C
	5′-CTTATCATCTTCGCAGCTCTGC
TAP2 1993	5′-TCTTTCTGAGGCACTGTGATC	283 bp	5′-TGATTGCTCACAGGCT-3′	54°C
	biotin-5′-GGAAACTCAAAGCAGGAAC

SNP: single nucleotide polymorphism.
PCR: polymerase chain reaction.
TAP: transporter associated with antigen processing.