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Disease Markers
Volume 35, Issue 5, Pages 573–579
http://dx.doi.org/10.1155/2013/392578
Research Article

CD90- (Thy-1-) High Selection Enhances Reprogramming Capacity of Murine Adipose-Derived Mesenchymal Stem Cells

1Department of Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
2Department of Frontier Science for Cancer and Chemotherapy, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
3Department of Surgery and Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima 737-0023, Japan
4Department of Complementary and Alternative Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

Received 18 June 2013; Revised 7 September 2013; Accepted 12 September 2013

Academic Editor: Chao Hung Hung

Copyright © 2013 Koichi Kawamoto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Mesenchymal stem cells (MSCs), including adipose tissue-derived mesenchymal stem cells (ADSC), are multipotent and can differentiate into various cell types possessing unique immunomodulatory features. Several clinical trials have demonstrated the safety and possible efficacy of MSCs in organ transplantation. Thus, stem cell therapy is promising for tolerance induction. In this study, we assessed the reprogramming capacity of murine ADSCs and found that CD90 (Thy-1), originally discovered as a thymocyte antigen, could be a useful marker for cell therapy. Method. Murine ADSCs were isolated from B6 mice, sorted using a FACSAria cell sorter by selection of or , and then transduced with four standard factors (4F; Oct4, Sox2, Klf4, and c-Myc). Results. Unsorted, -sorted, and -sorted murine ADSCs were reprogrammed using standard 4F transduction. ADSCs showed increased numbers of alkaline phosphatase-positive colonies compared with ADSCs. The relative reprogramming efficiencies of unsorted, -sorted, and -sorted ADSCs were 100%, 116.5%, and 74.7%, respectively. cells were more responsive to reprogramming. Conclusion. ADSCs had greater reprogramming capacity than ADSCs, suggesting that ADSCs have heterogeneous subpopulations. Thus, selection presents an effective strategy to isolate a highly suppressive subpopulation for stem cell-based tolerance induction therapy.