Mutation of NPM1 and FLT3 Genes in Acute Myeloid Leukemia and Their Association with Clinical and Immunophenotypic Features
Table 1
Clinical characteristics of AML patients with NPM1 and FLT3 mutations.
Total (161)
No mutation % (100)
NPM1 % (34)
FLT3/ITD % (35)
FLT3/D835 % (5)
FLT3 + NPM1 % (13)
Male : Female
110 : 51
69 : 31
20 : 14
24 : 11
4 : 1
7 : 6
Children : Adult
45 : 116
33 : 67
*4 : 30
8 : 27
2 : 3
2 : 11
M0/M1
24
15 (15)
12 (4)
17 (6)
40 (2)
23 (3)
M2
60
36 (36)
47 (16)
37 (13)
20 (1)
46 (6)
M3
9
5 (5)
6 (2)
6 (2)
0 (0)
0
M4
18
14 (14)
6 (2)
6 (2)
20 (1)
8 (1)
M5
26
16 (16)
15 (5)
17 (6)
20 (1)
15 (2)
M6
1
1 (1)
0 (0)
0 (0)
0 (0)
0
BAL
3
3 (3)
0 (0)
0 (0)
0 (0)
0
AML (NC)
20
10 (10)
15 (5)
17 (6)
0 (0)
8 (1)
MPO (+) stain
121
86 (63/73)
96 (24/25)
93 (28/30)
100 (2/2)
100 (9/9)
AR present
79
46 (46)
56 (19)
51 (18)
20 (1)
38 (5)
LAP
46
26 (26)
23 (8)
40 (14)
40 (2)
31 (4)
HSM
92
64 (64)
38 (13) (P = 0.01)
46 (16)
60 (3)
31 (4)
Hb level (g/dL)
6.2 (2–13.8)
6.2 (2.6–13.8)
6.6 (3–12.2)
6.2 (2–10.4)
5.3 (3.6–10.0) (
6.2 (3.6–10.1)
TLC (109/L)
31.5 (1–428)
27.4 (1–290)
34.7 (1.6–380)
60.2 (1.4–428) (P = 0.01)
41.6 (2.5–256)
48.7 (19–312) (P = 0.04)
Platelet (109/L)
22 (1–343)
20 (1–270)
46.5 (9–343) (P = 0.05)
23 (2–126)
20 (2–191)
37 (9–191)
Blast (%)
69 (10–98)
67.5 (17–98)
75 (10–95)
70 (29–96)
80 (50–90)
60 (29–92)
CR
83 (24/29)
52 (15/29)
73 (8/11)
29 (2/7)
100 (1)
50 (1/2)
P values were obtained on comparing each of FLT3 (ITD/D835), NPM1, and FLT3 + NPM1 mutation groups with the no mutation group (column 2). Mann-Whitney rank sum test was used for the difference in median variable of Hb, Plt, WBC, and blast parameters. For Hb, Plt, WBC, and blast, numbers represent median values while, numbers in parenthesis indicate range. For other categorical variables, either Fischer’s exact or Pearson’s test is used. P indicated P value. CR indicated complete remission after induction chemotherapy. Abbreviations: AML (NC): AML not classified, BAL: biphenotypic acute leukemia, AR: auer rod, MPO: myeloperoxidase, LAP: lymphadenopathy, and HSM: hepatosplenomegaly. *Bold significant P value = 0.02.
