Mutation of NPM1 and FLT3 Genes in Acute Myeloid Leukemia and Their Association with Clinical and Immunophenotypic Features

Table 2

Sequencing analysis of different patterns of NPM1 mutation in AML patients.

Sample ID	DNA sequence of exon 12 of NPM1 gene
Wild type	GATCTCTG —GCAG TGGAGGAAGTCTCTTTAAGAAAATAG
UH542	GATCTCTGTCTGGCAG TGGAGGAAGTCTCTTTAAGAAAATAG
UH545	GATCTCTGCATGGCAGTGGAGGAAGTCTCTTTAAGAAAATAG
UH533	GATCTCTG TTTGGCAG TGGAGGAAGTCTCTTTAAGAAAATAG
UH581	GATC CGCTTCTGGCAG TGGAGGAAGTCTCTTTAAGAAAATAG
UH591	GATC TATGTCTGGCAG TGGAGGAAGTCTCTTTAAGAAAATAG
UH595	GATCTCTGTCTGGCAG TGGAGGAAGTCTCTTTAAGAAAATAG
UH576	GATCTCTGTCTGGCAG TGGAGGAAGTCTCTTTAAGAAAATAG
UH567	GATCTCTGTGTGGCAG TGGAGGAAGTCTCTTTAAGAAAATAG

Sequence highlighted in bold color represents inserted nucleotides in NPM1 gene. TCTG is the most common 4 bp nucleotide insertion in exon 12 of NPM1 gene.