Association of a FGFR-4 Gene Polymorphism with Bronchopulmonary Dysplasia and Neonatal Respiratory Distress

Table 2

PCR conditions: primers, annealing temperatures, number of cycles and restriction enzymes (RE) for RFLP analysis.

Gene	Polymorphism	Forward-primer	Reverse-primer	PCR	RE
MMP-1	rs7125062	5′-ATC TCT GTC GGC AAA TTC GT-3′	5′-AGC AAC AAG AAG GAG CTG GA-3′	56.5°C, 35 cycl.	BseG1
MMP-2	rs7201	5′-TGC CCA AGA ATA GAT GCT GA-3′	5′-CTG TGA GCC ACA GAA GGT TG-3′	59.1°C, 35 cycl.	MboI
	rs243865	5′-CTG ACC CCC AGT CCT ATC TG-3′	5′-GAG CTG AGA CCT GAA GAG CCA AA-3′	59.1°C, 40 cycl.	BstXI
	rs17301608	5′-GCA GTG TAG ACA CTA GAG GAA GGA T-3′	5′-GTC ACC AAG GCC TGT ACC AC-3′	59.1°C, 40 cycl.	DpnII
MMP-9	rs20544	5′-AGT GCC CTG AGG ACT AGA G-3′	5′-CAT ACA TGC ATA CAT ACG TGC ATA C-3′	54.2°C, 40 cycl.	SacI
	rs3918242	5′-GCC TGG CAC ATA GTA GGC CC-3′	5′-CTT CCT AGC CAG CCG GCA TC-3′	67°C, 40 cycl.	XceI
	rs17576	5′-AAT TCA CCC TCC CGC ACT CT-3′	5′-GTT TTG GGG GCC AAT ACA TGA-3′	56.5°C, 35 cycl.	SmaI
MMP-16	rs2664352	5′-GGT AGG CCT GCT TTG GTT CT-3′	5′-GCC TTC CAA ACT CCA CTC ACA T-3′	56.5°C, 35 cycl.	NlaIII
FGFR-2	rs2981579	5′-CCA CTC AAG TCC CTG CTT GT-3′	5′-GTG GGG ACT GAT CAG AGG AA-3′	56.5°C, 40 cycl.	AccI
	rs1219648	5′-GCA CGC CTA TTT TAC TTG ACA CGC-3′	5′-CAT TGT GGT GAT CCT TCA CG-3′	59.1°C, 40 cycl.	HhaI
FGFR-4	rs376618	5′-GGG GGT AAC TGT GCC TAT TCTA-3′	5′-TAG CAG GGA GTG AAG GGA TG-3′	56.5°C, 40 cycl.	XbaI
	rs1966265	5′-CAA AGG TGC ACG TGT AGC AG-3′	5′-TCC CAC CTC AGA AGC CAT AC-3′	56.5°C, 40 cycl.	BamHI
FGF-2	rs11938826	5′-GAT GAT TTC TCA GGG CCA AA-3′	5′-GCT TCA CAT GCC TAT TTG CTT-3′	59.1°C, 40 cycl.	AluI
FGF-3	rs11263591	5′-GGT GTG TGG GCT GAA GAG AT-3′	5′-CAC CCT GAC ACT CCT GGT CT-3′	61.8°C, 40 cycl.	TaqI
	rs10796856	5′-CTT CCC CTC CTA GGC TTC AG-3′	5′-GAA TGC CAG GTT AGC AAA GG-3′	59.1°C, 40 cycl.	HhaI
FGF-4	rs3740639	5′-AAA AAC ACA CCC GCA GAA CT-3′	5′-TGA AGG TCA CCC ACT TCC TC-3′	59.1°C, 40 cycl.	HinfI
FGF-7	rs16962440	5′-TGT CGA ACA CAG TGG TAC CTG-3′	5′-GCA AAA GGA AGA TGA GAA AAG AT-3′	56.5°C, 40 cycl.	EcoRV
FGF-7, C15 or f33	rs4316697	5′-TGG CTC TTT CCA GCA ATT AGA-3′	5′-CCC TTT CTC TCC CAG GTT CT-3′	59.1°C, 40 cycl.	TaiI
	rs10519230	5′-AAT GTG ACC GCA TAT TGG TG-3′	5′-CAT TTT GGG AAC TGG GGT AA-3′	56.5°C, 35 cycl.	TaqI
FGF-18	rs4559013	5′-GAG GCA CAG AAT GAG GAA GG-3′	5′-TAC CTG GGC TCT CAA CAT CC-3′	56.5°C, 35 cycl.	Alw26I
SIRPA	rs3828016	5′-TCA AAC CCA GGC TTT CTG AC-3′	5′-AAC CAA GGC TGG CAC ATA AG-3′	63°C, 40 cycl.	NlaIII
	rs4814734	5′-CTG TGT ACA TCC TCT TAA ATG GAC-3′	5′-TGC TGT GTG CCA GAG TAT GC-3′	59.1°C, 40 cycl.	HinfI
TTF-1	rs11628131	5′-TGC AGC CTC ATT CAG CTT TA-3′	5′-GGT TGG AGA GGA GGA AAG GA-3′	56.5°C, 40 cycl.	TaqI
	rs999460	5′-ACC TGG GCG AGA AGT GAG TA-3′	5′-AGA GAG GGG AGA AGG AAT GG-3′	56.5°C, 40 cycl.	SspI