Serum Anti-CCNY Autoantibody Is an Independent Prognosis Indicator for Postoperative Patients with Early-Stage Nonsmall-Cell Lung Carcinoma
Figure 2
(a) The recombinant vectors pET30a-CCNY-6His were certificated by restricted enzyme cutting. Lane M: DNA marker; Lane 1: recombinant vectors pET30a-CCNY-6His, Lane 2: NCO I single restriction enzyme digestion, Lanes 3: NCO I and Xho I double restriction enzyme digestion. (b) SDS-PAGE analysis was performed to identify the expression of CCNY protein. Lane M: protein molecular weight marker, Lane 1: the negative control of BL21 (DE3), Lane 2: the negative control of BL21 (DE3)/pET30a, Lane 3: CCNY protein expressed in BL21 (DE3)/pET30a. (c) The validation of CCNY autoantibody. Lane 1: positive control (Rabbit anti-CCNY polyclonal antibody), Lane 2: positive control (His-tag antibody), Lanes 3, 4, 5, and 6: the serum with positive CCNY autoantibody, Lanes 7 and 8: the serum with negative CCNY autoantibody, Lane 9: negative control.
