Fluvastatin Upregulates the α_1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERK/p38 MAPK Pathways

Figure 3

Effects of Flu on the levels of LTCC protein determined by western blot analysis. PDGF time-dependently inhibited LTCC expression, which could be prevented by fluvastatin. (a) Dose-effect response of PDGF stimulation on the LTCC expression in VSMCs. (b) Time-effect response of Flu on the LTCC expression incubated by PDGF. β-actin served as the loading control. Data was represented as means ± SE of 3 separate experiments conducted in triplicate. versus blank control and versus cells incubation with PDGF. LTCC: L-type calcium channel subunit; Flu: fluvastatin; and PDGF: platelet derived growth factor.

(a) LTCC expression was inhibited by PDGF 

(b) Flu prevented the downregulation of LTCC expression induced by PDGF-BB