Disease Markers

Research Article

Promoter Methylation of SFRP3 Is Frequent in Hepatocellular Carcinoma

Table 1

The primer and probe sequences used in this study.
Primer sequence ( )Primer nameAssay
GTGTTGTTTTGGGGTTTTGTATTTGTATGSFRP3 UFMSPCR
CTACCTCCCACCTAAAAAAAAACTCCACSFRP3 URMSPCR
TTGGGGTGGGTTTTTTAGTGAGGGGTBS01 FBS sequencing
AACAAAAAAAACRCTCAAAAAAAACCBS01 RBS sequencing
GGCGTTGTTTTGGGGTTTCGTATTCSFRP3 MFMSPCR, QMSP
TCCCGCCTAAAAAAAAACTCCGSFRP3 MRMSPCR, QMSP
CTCTACCCTCCAATACCprobeQMSP
TCCCGAGGCCATCGTTACTSFRP3 FQRT-PCR (SyBr)
AGGCTTACATTTACAGCGTTCACSFRP3 RQRT-PCR
Sequence of SFRP3 promoter:
aaaaaaaaagtccaagtgtattagagctgttagtttccacgttaacccttaaggagcaaagctcaagagttctaattccactaggtggggggggcgggaatagaaggaaaaaaccccttttccttgcttctggtggcctatttgtagtcat
gaacagcatttctttgtttctctctctctttttttttttttttaaaggcaatcctccccccacctcctcccccgcagttattgaaaatggagacctctgtagtcactagctctgggttgatatggctccaccgttgctcgcaggggtctgtgttttccg
ctacttggacaaagtgacattgcttaagcctttccccccaccaggtctgactttctgcagagccagtgattgcagaggaaaagctgtagtttgcttaaaggaaatacctccaggaaggagggtctcgggtgggttcccaagtggggaact
agggggacttttccgtagggaattggggtgggctcttcagtgaggggctaggggctcgtttctggggccaaagacgggttccctagtgtgagggcgcgctcgactcggcgctgtcttggggtctcgcactcgcacggcttcgcaccccac
cgcctgcgactcccaggccttctcttccccgggcgcccactccattctcgggaagagcagccggcactggagggcagagactgccccaggggcggagctccctctcaggcgggaggtaggaaagtgcagagccgcccgggcagagg
cacagacgtccctgcggggctcctcctgagcgtccctcctgccagccagggtcgcagccgcagcggcggccgcagctcttagcccacacaggacttgtaaactcttactgcacccttctctcccattaggagcttttcctccctccttccc
cccaacccctctgtcctcctcactttggggaatttaatgctttctttagcatctttttgtgtgcgtgatctaggggaggagacaccccagagctccaactagctctcagctgaattctacttttgtttttatgtcttcctcgcctcctctcgtgtcc
ctcttatctgactgatctgcgaagtctgatgcttctgccagagggagaggaataaatagatgttgctgcttccgaaggcttagacGTTGGGAAAGAGCAGCCTGGGCGGCAGGGGCGGTGGCTG
GAGCTCGGTAAAGCTCGTGGGACCCCATTGGGGGAATTTGATCCAAGGAAGCGGTGATTGCCGGGGGAGGAGAAGCTCCCAGATCCTTGTG
TCCACTTGCAGCGGGGGAGGCGGAGACGGCGGAGCGGGCCTTTTGGCGTCCACTGCGCGGCTGCACCCTGCCCCATCCTGCCGGGATC.