Prognostic and Biological Significance of MicroRNA-127 Expression in Human Breast Cancer

Figure 6

Silencing of miR-127 reverses malignant phenotypes of BC cells. (a) Western blot analysis of BCL-6 protein expression in stably transfected MCF-7/shBCL-6 (or MCF-7/shcontrol) and SK-BR-3/shBCL-6 (or SK-BR-3/shcontrol) cells. GAPDH was used as an internal control. (b) MTT analysis of growth in MCF-7/shBCL-6 (or MCF-7/shcontrol) and SK-BR-3/shBCL-6 (or SK-BR-3/shcontrol) cells. (c) The colony formation assay was performed as described in Materials and Methods section. The number of colonies was counted and compared. (d) Flow cytomeric detection of apoptosis in MCF-7/shBCL-6 (or MCF-7/shcontrol) and SK-BR-3/shBCL-6 (or SK-BR-3/shcontrol) cells. (e) Western blot analysis of the expression levels of cleaved caspase-3, total caspase-3, cleaved PARP, and total PARP proteins in MCF-7/shBCL-6 (or MCF-7/shcontrol) and SK-BR-3/shBCL-6 (or SK-BR-3/shcontrol) cells. GAPDH was used as an internal control. (f) Scratch wound healing analysis of migration in MCF-7/shBCL-6 (or MCF-7/shcontrol) and SK-BR-3/shBCL-6 (or SK-BR-3/shcontrol) cells. (g) Transwell invasion analysis of invasion in MCF-7/shBCL-6 (or MCF-7/shcontrol) and SK-BR-3/shBCL-6 (or SK-BR-3/shcontrol) cells. Each experiment was performed at least in triplicate. , versus control.

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