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Disease Markers
Volume 2014, Article ID 594093, 8 pages
http://dx.doi.org/10.1155/2014/594093
Research Article

Identification of Human Tissue Kallikrein 6 as a Potential Marker of Laryngeal Cancer Based on the Relevant Secretory/Releasing Protein Database

1Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China
2State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, P.O. Box 2258, Beijing 100021, China
3Department of Head and Neck Surgical Oncology, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China
4National Center of Biomedical Analysis, Beijing 100021, China

Received 14 November 2013; Revised 3 January 2014; Accepted 4 January 2014; Published 11 February 2014

Academic Editor: Olav Lapaire

Copyright © 2014 Ying Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1: The clinicopathological data of the laryngeal cancer cases involved in the primary tissue culture.

Table S2: The clinical characteristics of the 149 patients with laryngeal SCC used for ELISA.

Table S3: The clinical characteristics of the 70 patients as the sources of preoperative and postoperative blood samples.

Table S4: The protein identifications in all the CM samples.

Table S5: The list of proteins previously reported in the serum of head and neck cancer patients.

Table S6: Forty-nine candidate biomarkers of laryngeal SCC in the “secretory/releasing” database.

Table S7: The performance of KLK6 in distinguishing cancer cases from controls at selected cutoff points

Figure S1: Representative images of HE-stained sections of normal tissue (A) and matched tumor tissue (B) derived from one laryngeal SCC patient after 0 h and 48 h in vitro, respectively. Magnification 200×.

Figure S2: Cellular components significantly enriched using the BiNGO tool, with the Benjamini & Hochberg False Discovery Rate correction for multiple testing and all human annotations as the reference set. The GO terms and their significance (negative log of the P value) are separately displayed on the x- and y-axes. “Extracellular region part” and “cell surface” are highlighted in the red box. P values less than 0.01 were considered to be statistically significant.

Figure S3: ROC curves with regard to the plasma KLK6 concentration discriminating laryngeal SCC patients from controls. The discriminatory power of the cancer cases from healthy cases, benign cases, and non-cancer cases are shown in A, B, and C, respectively. The values of the area under the ROC curve (AUC) were 0.587 (95% CI: 0.519-0.654; P=0.014) for A, 0.567 (95% CI: 0.501-0.633; P=0.047) for B, and 0.576 (95% CI: 0.517-0.635; P=0.010) for C.

  1. Supplementary Tables
  2. Supplementary Figures