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Disease Markers
Volume 2014 (2014), Article ID 683757, 5 pages
http://dx.doi.org/10.1155/2014/683757
Research Article

The Angiogenic Activity of Ascites in the Course of Ovarian Cancer as a Marker of Disease Progression

1Department of Gynecological Oncology and Oncology, Medicover Hospital, Rzeczypospolitej 5, 02-972 Warsaw, Poland
2Department of Obstetrics and Gynecology, Institute of Mother and Child, Kasprzaka 17A, 01-211 Warsaw, Poland
3Department of General and Experimental Pathology, Warsaw Medical University, Pawińskiego 3C, 02-106 Warsaw, Poland
4Department of Pathology, Centre of Biostructure Research, Warsaw Medical University, Chałubińskiego 5, 02-004 Warsaw, Poland
5Department of Applied Pharmacy and Bioengineering, Warsaw Medical University, Warsaw, Poland
6Institute of Mother and Child, Kasprzaka 17A, 01-211 Warsaw, Poland
71st Department of Obstetrics and Gynecology, Warsaw Medical University, Pl. Starynkiewicza 1, 02-015 Warsaw, Poland
8Department of Surgical Oncology, Holy Family Hospital, Madalińskiego 25, 02-544 Warsaw, Poland
9Department of Oncology, National Institute of Oncology, Wawelska 15, 02-034 Warsaw, Poland
10Department of Histology and Embryology, Centre of Biostructure Research, Warsaw Medical University, Chałubińskiego 5, 02-004 Warsaw, Poland

Received 27 June 2013; Revised 15 October 2013; Accepted 21 October 2013; Published 23 January 2014

Academic Editor: Natacha Turck

Copyright © 2014 Krzysztof Gawrychowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ovarian cancer cells are able to create invasive implants in the peritoneum and their growth is directly associated with the angiogenetic potential. This effect is probably stimulated by vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8), which are both found in ascites. The aim of this study was to assess the influence of ascites produced by ovarian cancer on the angiogenesis. Peritoneal fluid was collected from patients with advanced ovarian cancer; cancer cells were separated from CD45+ leukocytes. Angiogenesis was assessed in mice, after intradermal injection of full cellular suspension together with supernatant or phosphate buffered saline, purified cancer cells suspension, or CD45+ leukocytes suspension. The angiogenesis index (AI) was assessed after 72 hours. VEGF and Il-8 were measured in the supernatant and cellular suspension. AI was the highest in the isolated cancer cells suspensions as well in the group stimulated with supernatant. Both VEGF and IL-8 were high in supernatants from ascites rich in cancer cells (>45%). A significant correlation was revealed between IL-8 concentration and AI. We conclude that ascites in patients with advanced ovarian cancer stimulates angiogenesis and this mechanism is dependent mostly on cancer cells activity and enhanced by cooperation with infiltrating leukocytes.