Disease Markers / 2014 / Article / Tab 1

Research Article

Accurate, Fast and Cost-Effective Diagnostic Test for Monosomy 1p36 Using Real-Time Quantitative PCR

Table 1

qPCR primer sequences, genomic location, and size of each amplicon.

MarkerChrPrimer nameSequence (5′ → 3′)Amplicon sizeGenomic location of amplicon (hg19)

PRKCZ a1PRKCZ-FACGGTGTGAGCATGAGGATACc1252,020,732-2,020,856
PRKCZ-RCAGAGGCTGAAGCAAATGAACc

SKI a1SKI-FAGCTGATTGGGGGTAGGCc852,221,777-2,221,861
SKI-RTCAGGCTGAGCAGTGCAGc

HMBS b11HMBS-FACGGCTCAGATAGCATACAAGd185118,963,676-118,963,860
HMBS-RATGCCTACCAACTGTGGGTCAd

Chr: chromosome; target gene; breference gene; cprimer sequences designed using Primer3 software, version 0.4.0 (http://frodo.wi.mit.edu/); dprimer sequences derived from Saugier-Veber et al. [22].

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