Accurate, Fast and Cost-Effective Diagnostic Test for Monosomy 1p36 Using Real-Time Quantitative PCR

Table 1

qPCR primer sequences, genomic location, and size of each amplicon.

Marker	Chr	Primer name	Sequence (5′ → 3′)	Amplicon size	Genomic location of amplicon (hg19)
PRKCZ ^a	1	PRKCZ-F	ACGGTGTGAGCATGAGGATAC^c	125	2,020,732-2,020,856
		PRKCZ-R	CAGAGGCTGAAGCAAATGAAC^c
SKI ^a	1	SKI-F	AGCTGATTGGGGGTAGGC^c	85	2,221,777-2,221,861
		SKI-R	TCAGGCTGAGCAGTGCAG^c
HMBS ^b	11	HMBS-F	ACGGCTCAGATAGCATACAAG^d	185	118,963,676-118,963,860
		HMBS-R	ATGCCTACCAACTGTGGGTCA^d

Chr: chromosome; atarget gene; ^breference gene; ^cprimer sequences designed using Primer3 software, version 0.4.0 (http://frodo.wi.mit.edu/); ^dprimer sequences derived from Saugier-Veber et al. [22].