Urine Annexin A1 as an Index for Glomerular Injury in Patients

Figure 4

Detection of ANXA1 and ANXA2 protein expression in kidney tissue by IHC at different time points after ADG induction. (a)–(f) ANXA1 and (g)–(l) ANXA2. Kidney tissue sections on day 0 ((a) and (g)), day 3 ((b) and (h)), day 7 ((c) and (i)), day 14 ((d) and (j)), or day 21 ((e) and (k)). The arrows indicate epithelial cells in the crescent-like formation of the glomerulus. Original magnification, ×400. (f) and (l) Semiquantitative analysis of cellular protein expression by IHC. Scoring was performed for the three major components of the parietal epithelial cells (solid bars), podocytes (open bars), and mesangial cells (hatched bars). Each bar represents the mean±SEM for six mice per group. ^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.005, compared to the normal controls (day 0). #: not detectable.