Review Article
BRCA Genetic Screening in Middle Eastern and North African: Mutational Spectrum and Founder BRCA1 Mutation (c.798_799delTT) in North African
Table 1
Details of studies examining BRCA1 in MENA.
| | Study | Case selection | Region covered | Detection method |
| Hadjisavvas et al. [19]
(Cyprus) | 40 familial cases | All | Sequencing |
| Loizidou et al. [20]
(Cyprus) | 26 familial cases | All | Sequencing |
|
Atoum and Al-Kayed [16]
(Jordan) | 135 BC females | Exons 2, 11, and 20 | DGGE |
| Jalkh et al. [21]
(Lebanon) | 72 familial cases | All | Sequencing |
|
Ibrahim et al. [17]
(Egypt) | 20 healthy females and 30 familial cases | c.68_69delAG/exon 2
c.181T>G/exon5
c.5266dupC/exon 20 | MS-PCR
RFLP |
|
El-Debaky et al. [18]
(Egypt) | 60 familial cases | Exons 2, 8, 13, and 22 | SSCP
Heteroduplex analysis
Sequencing |
| Tazzite et al. [22]
(Morocco) | 34 familial cases
6 single cases
(<40 years) | All | Sequencing |
| Laraqui et al. [23]
(Morocco) | 19 familial cases
102 early-onset sporadic (<45 years) | All | Sequencing |
| Uhrhammer et al. [24]
(Algeria) | 13 familial cases
51 young sporadic cases (<38 years) | All | Sequencing
MLPA |
| Cherbal et al. [25]
(Algeria) | 86 familial cases | All | HRM
Sequencing
MLPA |
| Troudi et al. [26]
(Tunisia) | 34 familial cases | All | Sequencing |
| Mahfoudh et al. [27]
(Tunisia) | 16 familial cases | All | Sequencing |
| | Riahi et al. [28] | 48 familial cases | All | Sequencing |
|
|
DGGE: denaturing gradient electrophoresis; MS-PCR: mutagenically separated PCR, SSCP: single-strand conformation polymorphism, RFLP: restriction fragment length polymorphism, HRM: high-resolution melting, MLPA: multiplex ligation-dependent probe amplification.
|
