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Disease Markers
Volume 2015, Article ID 316182, 9 pages
http://dx.doi.org/10.1155/2015/316182
Research Article

The Expression of Notch/Notch Ligand, IL-35, IL-17, and Th17/Treg in Preeclampsia

1Department of Obstetrics, Maternity and Child Health Hospital of Zhenjiang, No. 20 Zhengdong Road, Zhenjiang, Jiangsu 212001, China
2Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China
3Central Laboratory of Medicine, Maternity and Child Health Hospital of Zhenjiang, No. 20 Zhengdong Road, Zhenjiang, Jiangsu 212001, China
4Obstetrics and Gynecology, The Affiliated People Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, China
5Laboratory of Medicine, Maternity and Child Health Hospital of Zhenjiang, No. 20 Zhengdong Road, Zhenjiang, Jiangsu 212001, China

Received 13 February 2015; Revised 20 April 2015; Accepted 27 April 2015

Academic Editor: Maddalena Ruggieri

Copyright © 2015 Weiping Cao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to examine the interaction of Notch/Notch ligand with Th17/Treg, cytokines IL-35 and IL-17 in cases of preeclampsia (PE). Methods. Peripheral blood was obtained from 42 PE patients and 22 health pregnant women. The mRNA expressions of Notch/Notch ligand, Treg transcription factor FoxP3 and Th17 transcription factor RORγt, EBI3 and P35 (IL-35 two subunits), and IL-17 were determined by qPCR. The serum levels of IL-17 and IL-35 were measured by ELISA. Results. It was observed that the expressions of Foxp3, EBI3, and P35 in PE patients were lower compared with normal pregnancy, whereas the RORγt expression was significantly increased. The results also demonstrated that PE patients exhibited decreased levels of Treg-related cytokine IL-35, whereas IL-17 was significantly increased. PE patients expressed higher levels of Notch receptor (1–4) and Notch ligand of DLL4, whereas Notch ligand of Jagged-1, -2 was much lower. Furthermore, the levels of FoxP3 T cells correlated positively with Jagged-2. In addition, there were positive correlations between the mRNA level of IL-17 and DLL4. Conclusion. Our results indicated that maternal immunological changes may reverse maternal tolerance in PE, and this phenomenon may due to the Th17/Treg imbalance affected by Notch/Notch ligand.