Hypermethylation-Associated Silencing of miR-125a and miR-125b: A Potential Marker in Colorectal Cancer

Figure 2

Bisulfite sequencing analysis of miR-125 in CRC and adjacent nontumor tissues. (a) Schematic diagrams show the distribution of CpG dinucleotides in the promoter region of miR-125a and miR-125b. (b) BSP of the upstream regulatory region of miR-125 was performed for representative tissues. For each sample, at least five separate clones were sequenced, and the results are shown here. Unmethylated CpG sites are shown as open circles, whereas methylated CpG sites are indicated by closed circles. For each row of circles, the sequence results for an individual clone of the bisulfite-PCR product are given. The number of methylated CpGs divided by the total number of true CpGs analyzed is given as a percentage on the right of each BSP result. (c) The methylated levels of miR-125a and miR-125b were significantly higher in tumor tissues compared with adjacent nontumor tissues. The results were expressed as mean ± SD. .

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