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Disease Markers
Volume 2015 (2015), Article ID 365845, 6 pages
http://dx.doi.org/10.1155/2015/365845
Research Article

Polymorphisms rs12998 and rs5780218 in KiSS1 Suppressor Metastasis Gene in Mexican Patients with Breast Cancer

1División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico
2Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada, No. 950, Colonia Independencia, 44340 Guadalajara, JAL, Mexico
3Laboratorio de Inmunología, Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada, No. 950, Colonia Independencia, 44340 Guadalajara, JAL, Mexico
4División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico
5IMAREFI, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ing. Ramón Padilla Sánchez, No. 2100, Nextipac, 44600 Zapopan, JAL, Mexico

Received 10 July 2014; Revised 26 January 2015; Accepted 4 February 2015

Academic Editor: Natacha Turck

Copyright © 2015 Edhit Guadalupe Cruz Quevedo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. KiSS1 is a metastasis suppressor gene associated with inhibition of cellular chemotaxis and invasion attenuating the metastasis in melanoma and breast cancer cell lines. Along the KiSS-1 gene at least 294 SNPs have been described; however the association of these polymorphisms as genetic markers for metastasis in breast cancer studies has not been investigated. Here we describe two simple PCR-RFLPs protocols to identify the rs5780218 (9DelT) and the rs12998 (E20K) KiSS1 polymorphisms and the allelic, genotypic, and haplotypic frequencies in Mexican general population (GP) and patients with benign breast disease (BBD) or breast cancer (BC). Results. The rs5780218 polymorphism was individually associated with breast cancer and the rs12998 polymorphism shows statistically significant differences when GP versus case (BC and BBD) groups were compared . The H1 Haplotype (G/-) occurred more frequently in BC group (0.4256) whereas H2 haplotype (G/T) was the most prevalent in BBD group (0.4674). Conclusions. Our data indicated that the rs5780218 polymorphism individually confers susceptibility for development of breast cancer in Mexican population and a possible role as a genetic marker in breast cancer metastasis for H1 haplotype (Wt/variant) in KiSS1 gene must be analyzed in other populations.