Research Article
Clinical Implications and Prognostic Values of Prostate Cancer Susceptibility Candidate Methylation in Primary Nonmuscle Invasive Bladder Cancer
Table 2
PRAC primers used for pyrosequencing analysis.
| ||||||||||||||||||||
Primer was designed using NCBI Reference Sequences build version 36.1. The PCR reaction contained 0.01 μM each of primers and Bioneer Taq (Bioneer, Daejeon, Korea), and 20 ng of bisulfite-treated DNA. The thermocycling parameters were as follows: denaturation at 94°C for 5 minutes, followed by 45 cycles at 94°C for 30 seconds, annealing at 55°C for 30 seconds, 72°C for 30 seconds, and a final extension at 72°C for 5 minutes. bp: base pairs; PRAC: prostate cancer susceptibility candidate; TSS: transcription start site. |