Krüppel-Like Factor 4 Inhibits the Transforming Growth Factor-β1-Promoted Epithelial-to-Mesenchymal Transition via Downregulating Plasminogen Activator Inhibitor-1 in Lung Epithelial Cells

Figure 2

TGF-β promotes PAI-1 and induces epithelial-to-mesenchymal transition (EMT) in LA-4 cells. (a) Western blot analysis of PAI-1 and EMT-associated markers in LA-4 cells which were treated with 0, 1, 2, 5, or 10 ng/mL TGF-β for 24 hours. (b–e) Relative levels of PAI-1 (b), E-cadherin (c), Collagen I (d), and α-SMA (e) to β-actin in the LA-4 cells, which were treated with 0, 1, 2, 5, or 10 ng/mL TGF-β for 24 hours; each result was averaged for three independent experiments. Statistical significance was shown as , , , or ns: no significance.

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