Krüppel-Like Factor 4 Inhibits the Transforming Growth Factor-β1-Promoted Epithelial-to-Mesenchymal Transition via Downregulating Plasminogen Activator Inhibitor-1 in Lung Epithelial Cells

Figure 4

KLF-4 in both mRNA and protein levels in LA-4 cells, which were infected with KLF-4-overexpressed adenovirus. (a) The mRNA level of KLF-4 in LA-4 cells which were infected with 0, 1, or 5 multiplicity of infection (MOI) KLF-4-overexpressed (Ad-KLF-4) or control (Ad-Con) adenovirus for 12 hours. (b) KLF-4 mRNA level in LA-4 cells which were infected with 5 MOI Ad-KLF-4 or Ad-Con for 0, 3, 6, or 12 hours. (c) and (d) Protein levels of KLF-4 in LA-4 cells which were infected with 0, 1, or 5 MOI Ad-KLF-4 or Ad-Con virus. The protein level of KLF-4 was normalized to β-actin. Data represent the mean ± SEM for triple independent experiments. , , , or ns: no significance.

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