Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers
Table 1
The effect of gender and blood contamination on oxidative stress markers was analyzed using two-way RM ANOVA. Separate ANOVAs were computed for whole blood, plasma, red blood cells (RBC), and hemoglobin contamination.
Two-way RM ANOVA
value
value summary
Salivary AOPP
Blood contamination
Effect of blood
49.86
<0.0001
Effect of gender
0.51
0.49
ns
Plasma contamination
Effect of plasma
33.59
<0.0001
Effect of gender
3.68
0.09
ns
RBC contamination
Effect of RBC
296.3
<0.0001
Effect of gender
0.20
0.66
ns
Hemoglobin contamination
Effect of hemoglobin
28.31
<0.0001
Effect of gender
1.36
0.28
ns
Salivary AGEs
Blood contamination
Effect of blood
21.39
<0.0001
Effect of gender
5.42
0.0483
Plasma contamination
Effect of plasma
20.75
<0.0001
Effect of gender
4.48
0.07
ns
RBC contamination
Effect of RBC
68.02
<0.0001
Effect of gender
5.25
0.05
ns
Hemoglobin contamination
Effect of hemoglobin
19.17
<0.0001
Effect of gender
6.52
0.0340
Salivary FRAS
Blood contamination
Effect of blood
12.00
<0.0001
Effect of gender
0.99
ns
Plasma contamination
Effect of plasma
23.59
<0.0001
Effect of gender
0.27
0.62
ns
RBC contamination
Effect of RBC
9.772
<0.0001
Effect of gender
0.18
0.68
ns
Hemoglobin contamination
Effect of hemoglobin
18.32
<0.0001
Effect of gender
0.8376
0.39
ns
Salivary TAC
Blood contamination
Effect of blood
24.31
<0.0001
Effect of gender
0.20
0.67
ns
Plasma contamination
Effect of plasma
33.02
<0.0001
Effect of gender
0.51
0.49
ns
RBC contamination
Effect of RBC
24.37
<0.0001
Effect of gender
0.25
0.63
ns
Hemoglobin contamination
Effect of hemoglobin
43.80
<0.0001
Effect of gender
0.15
0.71
ns
AOPP, advanced oxidation protein products; AGEs, advanced glycation end products; FRAP, ferric reducing antioxidant power; TAC, total antioxidant capacity; ns, nonsignificant. ; .
