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Research Article

Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers

Table 1

The effect of gender and blood contamination on oxidative stress markers was analyzed using two-way RM ANOVA. Separate ANOVAs were computed for whole blood, plasma, red blood cells (RBC), and hemoglobin contamination.

Two-way RM ANOVA  
value value summary

Salivary AOPP
Blood contamination
 Effect of blood49.86<0.0001
 Effect of gender0.510.49ns
Plasma contamination
 Effect of plasma33.59<0.0001
 Effect of gender3.680.09ns
RBC contamination
 Effect of RBC296.3<0.0001
 Effect of gender0.200.66ns
Hemoglobin contamination
 Effect of hemoglobin28.31<0.0001
 Effect of gender1.360.28ns

Salivary AGEs
Blood contamination
 Effect of blood21.39<0.0001
 Effect of gender5.420.0483
Plasma contamination
 Effect of plasma20.75<0.0001
 Effect of gender4.480.07ns
RBC contamination
 Effect of RBC68.02<0.0001
 Effect of gender5.250.05ns
Hemoglobin contamination
 Effect of hemoglobin19.17<0.0001
 Effect of gender6.520.0340

Salivary FRAS
Blood contamination
 Effect of blood12.00<0.0001
 Effect of gender0.99ns
Plasma contamination
 Effect of plasma23.59<0.0001
 Effect of gender0.270.62ns
RBC contamination
 Effect of RBC9.772<0.0001
 Effect of gender0.180.68ns
Hemoglobin contamination
 Effect of hemoglobin18.32<0.0001
 Effect of gender0.83760.39ns

Salivary TAC
Blood contamination
 Effect of blood24.31<0.0001
 Effect of gender0.200.67ns
Plasma contamination
 Effect of plasma33.02<0.0001
 Effect of gender0.510.49ns
RBC contamination
 Effect of RBC24.37<0.0001
 Effect of gender0.250.63ns
Hemoglobin contamination
 Effect of hemoglobin43.80<0.0001
 Effect of gender0.150.71ns

AOPP, advanced oxidation protein products; AGEs, advanced glycation end products; FRAP, ferric reducing antioxidant power; TAC, total antioxidant capacity; ns, nonsignificant.
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