Table of Contents Author Guidelines Submit a Manuscript
Disease Markers
Volume 2015, Article ID 543282, 7 pages
http://dx.doi.org/10.1155/2015/543282
Research Article

Serum Enzyme Profiles Differentiate Five Types of Muscular Dystrophy

1Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, China
2Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, China
3Department of Healthcare Clinic, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, China
4Department of Laboratory Medicine, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, China
5Department of Epidemiology and Health Statistics, School of Public Health, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, China

Received 16 February 2015; Revised 9 April 2015; Accepted 15 April 2015

Academic Editor: Donald H. Chace

Copyright © 2015 Yuling Zhu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Differentiation among types of muscular dystrophy (MD) has remained challenging. In this retrospective study, we sought to develop a methodology for differentiation of MD types using analysis of serum enzyme profiles. Methods. The serum levels of enzymes from 232 patients, including 120 with DMD, 36 with BMD, 36 with FSHD, 46 with LGMD, and 11 with EDMD, were evaluated. Results. The characteristic profiles of serum enzymes facilitated differentiation of these five types of MD. DMD was characterized by simultaneous elevation of ALT, AST, LDH, and ALP; BMD and LGMD were characterized by elevation of ALT, AST, and LDH; and FSHD and EDMD were characterized by a lack of abnormal serum enzyme levels. We further developed discriminant functions to distinguish BMD and LGMD. For LGMD, LGMD2B patients had significantly higher ALP levels than non-LGMD2B patients ( U/L versus  U/L, resp., ). Conclusions. Our approach enabled the determination of MD subtypes using serum enzyme profiles prior to genetic testing, which will increase the chance a mutation will be found in the first gene analyzed.