Upregulated Expression of SOX4 Is Associated with Tumor Growth and Metastasis in Nasopharyngeal Carcinoma

Figure 3

The expression of SOX4 in proliferating CNE2 cells. (a) Flow cytometry was used to quantify the cell cycle progress in CNE2 cells. The cells were subjected to serum starvation for 72 h (S72 h) and then progressed into cell cycle by refeeding medium containing 10% FBS for 4, 8, 12, 24, and 36 h (R4 h, R8 h, R12 h, R24 h, and R36 h). (b) Western blot was used to investigate the expression of SOX4 and PCNA in CNE2 cells that were subjected to serum starvation and refeeding. β-actin was used as a control for protein load and integrity. (c) The bar demonstrated the ratio of SOX4 and PCNA protein expression to β-actin by densitometry. The data shown were representative of at least three independent experiments. Data were analyzed by Student’s -test. .

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