3-Nitrotyrosine Modified Proteins in Atherosclerosis

Table 1

Quantitative estimation of 3-nitrotyrosine in CVD plasma.

	Control		CVD		Change (%)	Reference
	NO2-Tyr/Tyr; (μmol/mol)^1	NO2-Tyr/protein molecule^2	NO2-Tyr/Tyr; (μmol/mol)^1	NO2-Tyr/protein molecule^2
Total serum proteins^3	6.1 (3.9–7.8)	NA	9.0 (5.7–12.9)	NA	47	[57]
ApoA-I^3	438 (335–598)	1/325	629 (431–876)	1/227	44	[57]
ApoB-100^3	4.0 (1.3–6.9)	1/1,644	8.7 (5.2–12.1)	1/756	117	[57]
Fibrinogen^4	24.6 (23.4–25.9)	1/303	31.8 (28.7–34.9)	1/235	29	[56]

 ¹Nitrotyrosine levels are reported as median (IQR).
²The number of Tyr modified residues per protein molecule (NO₂-Tyr/protein molecule) was calculated using 153, 7, and 134 Tyr residues for apoB-100, apoA-1, and fibrinogen, respectively, from the PromParam tool (Expasy) [76].
³Data in reference [57] were obtained using stable isotope dilution LC/ESI/MS/MS.
⁴Plasma concentrations of total and nitrated fibrinogen in reference [56] were determined by ELISA and reported as mg/mL for fibrinogen and nM for nitrotyrosine; mol NO₂-Tyr/mol Tyr were calculated using a molecular weight for fibrinogen of 340 kDa and 134 Tyr residues.