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Disease Markers
Volume 2015, Article ID 828145, 11 pages
http://dx.doi.org/10.1155/2015/828145
Research Article

Identification of Differentially Expressed Genes Associated with Prognosis of B Acute Lymphoblastic Leukemia

1Departamento de Bioquimica y Medicina Molecular, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico
2Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km 6, 98160 Ejido la Escondida, ZAC, Mexico
3Servicio de Hematologia, Hospital Universitario “Jose Eleuterio Gonzalez”, Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico
4Instituto Nacional de Medicina Genomica (INMEGEN), Periferico Sur No. 4809, Col. Arenal Tepepan, Delegacion Tlalpan, 14610 Mexico, DF, Mexico
5Mesoamerican Center of Public Health Studies and Disasters (CEMESAD), Universidad Autonoma de Chiapas (UNACH), Avenida Pista Principal esq Pista Secundaria, S/N, 30798 Tapachula, CHIS, Mexico
6Centro de Investigacion y Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo Leon, Carlos Canseco, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico

Received 31 October 2014; Revised 27 January 2015; Accepted 30 January 2015

Academic Editor: Olav Lapaire

Copyright © 2015 Idalia Garza-Veloz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Acute lymphoblastic leukemia type B (B-ALL) is a neoplastic disorder with high mortality rates. The aim of this study was to validate the expression profile of 45 genes associated with signaling pathways involved in leukemia and to evaluate their association with the prognosis of B-ALL. Methods. 219 samples of peripheral blood mononuclear cells obtained from 73 B-ALL patients were studied at diagnosis, four, and eight weeks after starting treatment. Gene expression was analyzed by quantitative real-time polymerase chain reaction. Results. Normalized delta Cq values of 23 genes showed differences between B-ALL and controls at diagnosis time ( values < 0.05). There were significant associations between B-ALL patients relapse/death and the expression levels of IL2RA, SORT1, DEFA1, and FLT3 genes at least in one of the times evaluated ( values < 0.05 and odds ratio ranges: 3.73–27). The association between FLT3 deregulation and relapse/death was a constant in the times studied and their overexpression significantly increased the odds of relapse/death in a range of 3.73 and 6.05 among study population ( values < 0.05). Conclusions. Overexpression of FLT3 and DEFA1 genes retained independent prognostic significance for B-ALL outcome, reflected as increased risks of relapse/death among the study population.