MHC Class I Chain-Related Gene A Diversity in Patients with Cutaneous Malignant Melanoma from Southeastern Spain

Campillo, José Antonio; López-Hernández, Ruth; Martínez-Banaclocha, Helios; Bolarín, José Miguel; Gimeno, Lourdes; Mrowiec, Anna; López, Manuela; Heras, Beatriz Las; Minguela, Alfredo; Moya-Quiles, Maria Rosa; Legáz, Isabel; Frías-Iniesta, José Francisco; García-Alonso, Ana María; Álvarez-López, María Rocío; Martínez-Escribano, Jorge Antonio; Muro, Manuel

doi:https://doi.org/10.1155/2015/831864

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Research Article | Open Access

Volume 2015 | Article ID 831864 | https://doi.org/10.1155/2015/831864

MHC Class I Chain-Related Gene A Diversity in Patients with Cutaneous Malignant Melanoma from Southeastern Spain

José Antonio Campillo,¹Ruth López-Hernández,¹Helios Martínez-Banaclocha,¹José Miguel Bolarín,¹Lourdes Gimeno,¹Anna Mrowiec,¹Manuela López,¹Beatriz Las Heras,¹Alfredo Minguela,¹Maria Rosa Moya-Quiles,¹Isabel Legáz,^1,2and José Francisco Frías-Iniesta³ et al.

Academic Editor: Giuseppe Murdaca

Received01 Jan 2015

Accepted19 Feb 2015

Published09 Mar 2015

Abstract

A limited number of studies have been performed so far on the polymorphism in the transmembrane region (exon 5) of the major histocompatibility complex class I chain-related gene A (MICA) in patients with melanoma. However, the influence of MICA polymorphism in extracellular domains (exons 2, 3, and 4) has not been investigated on melanoma disease. This study aims to characterize the influence of extracellular MICA polymorphism, and its previously described linkage disequilibrium with HLA-B locus, on patients with cutaneous melanoma from southeastern Spain. For this purpose, MICA and HLA-B genotyping was performed in 233 patients and 200 ethnically matched controls by luminex technology. Patients were classified according to the presence of methionine or valine at codon 129 of MICA gene. We found a high frequency of MICA^*009 in melanoma patients compared with controls (, Pc = 0.03). Our results also showed an association between MICA^*009 and HLA-B^*51 alleles in both patients and controls. This association was stronger in patients than controls (). However, a multivariate logistic regression model showed that neither MICA^*009 nor the combination MICA^*009/HLA-B^*51 was associated with melanoma susceptibility. No relationship was observed between MICA-129 dimorphism and melanoma nor when MICA polymorphism was evaluated according to clinical findings at diagnosis.

1. Introduction

Natural killer (NK) and antigen-specific cytolytic CD8⁺ T cells are believed to be involved in the immune response against melanoma [1–3], a tumor characterized by a poor clinical outcome [4]. Although melanoma is considered one of the most immunogenic tumors [2], it can escape from the host immune surveillance, especially if a state of tolerance is induced [5–7].

The effector function of NK and CD8⁺ T cells may be regulated by a balance between stimulatory and suppressing signals. These signals are mediated by a group of receptors after the interaction with their cognate ligands expressed on tumor cells [8–10], including tumoral melanocytes [1, 11]. In this context, it should be noted that killing of melanoma cells by NK cells is controlled by multiple activating receptor-ligand interactions [3, 12, 13], of which the NKG2D/NKG2D ligand system seems to be the major entity responsible for the lysis of melanoma tumor cells [3].

In humans, ligands of NKG2D activating receptor include the major histocompatibility complex class I chain-related (MIC) and UL16 binding protein molecules [14–16]. MIC genes include seven members (MICA-MICG), five of which are pseudogenes and the other two (MICA and MICB) are functional genes closely related to each other [15]. Unlike human leukocyte antigen (HLA) classical class I genes, MICA/B genes encode for stress-induced cell-surface molecules which do not associate with -microglobulin and do not seem to present peptides [17]. MICA/B proteins have a limited tissue distribution [17, 18], as they are mainly expressed in gastrointestinal epithelium, keratinocytes, endothelial cells, monocytes and fibroblasts, renal and pancreatic allograft, and lesions and cell lines derived from both epithelial tumors and cutaneous melanoma [1, 11, 19].

MICA gene is located in the HLA class I region of chromosome 6, 46 kb centromeric to the HLA-B locus, and is by far the most divergent mammalian MIC gene known [20]. It displays a high degree of allelic polymorphism in exon 5, encoding the transmembrane region [21], and in exons 2, 3, and 4, encoding the three extracellular domains alfa-1, alfa-2, and alfa-3 of the MICA protein, respectively [22]. Although the functional significance of MICA gene diversity is unknown, certain changes in the amino acid sequence of MICA proteins could influence the affinity with its cognate NKG2D receptor [23]. Thus, it has been demonstrated that the presence of methionine (Met) or valine (Val) at codon 129 of the alfa-2 domain may confer strong or weak affinity, respectively.

MICA polymorphism has been associated with a variety of diseases [24], including inflammatory and autoimmune diseases [25, 26], infectious and tumor diseases [27, 28], or even chronic graft-versus-host disease [29]. Remarkably, a limited number of studies concerning the polymorphism in MICA gene transmembrane region have been carried out in melanoma disease [30, 31]. However, to date, no studies have been performed on MICA polymorphism in the extracellular domains in melanoma. Thus, the aim of the present study was to determine whether extracellular MICA polymorphism and its previously described linkage disequilibrium with HLA-B locus [22, 32, 33] may influence the susceptibility to cutaneous melanoma in a group of patients from Murcia, a Mediterranean Spanish Region.

2. Materials and Methods

2.1. Subjects

A total of 233 Caucasian patients with histological diagnosis of cutaneous malignant melanoma were recruited between 1996 and 2013 from the Dermatology Department of University Clinical Hospital Virgen de la Arrixaca in Murcia, Spain. Patient classification was made according to the American Joint Committee on Cancer (AJCC) recommendations for cutaneous melanoma [34]. Melanoma patient age was (mean ± SD) years old (112 men and 121 women). Exclusion criteria were history of another malignant, autoimmune, inflammatory, or infectious chronic disease, or immunodeficiency. Additionally, a series of 200 sex- and age-matched Caucasian healthy individuals of the same ethnic origin was also studied. This series consisted of 92 men and 108 women, aged (mean ± SD). Demographic data of the enrolled subjects are summarized in Table 1.

All individuals included in the present study were unrelated and randomly selected. In all cases, informed consent was obtained and the protocol of study was approved by the institutional ethical committee, in accordance with the Declaration of Helsinki Principles.

2.2. Sample Collection and DNA Preparation

Blood samples were obtained by venipuncture from patients and healthy individuals and collected in anticoagulated vacutainer tubes (Becton Dickinson, Mountain View CA, USA). Genomic DNA was extracted by using the QIAamp DNA Blood Midi Kit (QIAGEN, Hilden, Germany), as recommended by the manufacturer.

2.3. MICA and HLA-B Genotyping

The samples were typed for MICA and HLA-B alleles using the polymerase chain reaction-specific oligonucleotide sequence (PCR-SSO) method by microbeads array luminex technology (One Lambda, CA). Reactions were read with the LABScan 100 flow cytometry analyzer and assignment of allelic types was performed using the HLA Fusion 2.0 software (One Lambda, CA). Data of MICA genotyping were also used to classify patients and controls based on the presence of valine (val) or methionine (met) amino acids at codon 129 of the alfa-2 domain.

2.4. Statistical Analysis

Demographic data and analysis results were collected in a database (Microsoft Access 11.0; Microsoft corporation, Seattle, WA), and statistical analysis was performed using SPSS 15.0 software (SPSS Inc., Chicago IL, USA). Chi-square test and two-tailed unpaired -test were used to detect differences regarding sex and age, respectively. MICA allele frequencies were estimated by direct counting and represent the percentage of individuals positive for a certain allele. Associations between MICA gene diversity and melanoma disease were established by comparing MICA allele frequencies in groups of patients with each other, and with healthy controls, using chi-square and two-sided Fisher’s exact tests. Data were also analyzed by using a multivariate logistic regression model. Odds ratio (OR) and its 95% confidence interval (CI) were calculated to estimate any relative risk. value <0.05 was considered significant. Corrected value (Pc) was obtained by multiplying the value by the number of alleles tested according to Bonferroni’s correction [35]. The power of the statistical analysis was calculated by Post-hoc Power calculator as described (http://clincalc.com/stats/power.aspx).

3. Results

3.1. Clinical and Histological Characteristics of Patients with Cutaneous Malignant Melanoma

As summarized in Table 1, 162 patients presented histological diagnosis of superficial spreading melanoma (SSM), 29 of nodular melanoma (NM), 16 of lentigo maligna melanoma (LMM), 13 of acral lentiginous melanoma (ALM), 11 of melanoma “in situ,” 1 of desmoplastic melanoma (DM), and 1 of spitzoid melanoma (SM).

Patients were also classified according to tumor thickness: ≤1.0 mm and >1.0 mm; the presence or absence of ulceration of primary lesion, and the presence or absence of sentinel lymph node (SLN) metastasis at diagnosis. Data about tumor thickness and about ulceration were available for 232 and 217 out of 233 patients, respectively, while information about SLN biopsy was available for 141 out of 233 patients, 119 of them showing benign SLN (Table 1).

3.2. MICA and Melanoma Disease

The frequency of MICA alleles in melanoma patients compared with a control group is summarized in Table 2. MICA^*009 allele frequency was found to be significantly increased in patients compared with controls (, OR = 2.14; 95% CI, 1.31–3.49; Post-hoc Power = 92%); this association remained statistically significant after -correction for multiple testing (Pc = 0.03). When the two most frequent histological subtypes of melanoma, such as SSM and NM, were individually analyzed, a significant increase in the frequency of MICA^*009 allele was observed in both groups of patients compared with controls (25% in SSM and 35% in NM versus 15% in controls; and , resp.).

An additional analysis was performed based on the previous linkage disequilibrium reported between MICA^*009 and HLA-B^*51 alleles [22, 32, 33]. In a first analysis this study showed an association between MICA^*009 and HLA-B^*51 in patients and control group (Table 3). This association was stronger in the patients because 50% of MICA^*009 positive patients coexpressed HLA-B^*51 against 41% of MICA^*009 positive controls. In fact, the frequency of individuals carrying MICA^*009 and HLA-B^*51 was significantly higher in patients than in control group (13% versus 6%; ), as shown in Table 3. However, when a multivariate logistic regression analysis was performed, the results showed that neither MICA^*009 nor the combination MICA^*009/HLA-B^*51 was associated with melanoma susceptibility (Table 4).

On the other hand, a decreased frequency of MICA^*002 was found in melanoma patients compared with controls (, Pc = 0.07; OR = 0.50, 95% CI, 0.32–0.80; Post-hoc Power = 79%), as shown in Table 2. Likewise, MICA^*010 and MICA^*019 frequencies were also underrepresented in patients compared with controls (, Pc = 0.37; OR = 0.27, 95% CI, 0.09–0.86; Post-hoc Power = 58% and , Pc = 0.24; OR = 0.10, 95% CI, 0.01–0.83; Post-hoc Power = 74%, resp.), but significances were lost after -correction.

The distribution of MICA allele frequencies according to the dimorphism at position 129, and their genotypes, was also analyzed, but no significant differences were found between patients and controls, as shown in Table 5.

The influence of MICA polymorphism on melanoma was further evaluated according to clinical findings at diagnosis such as tumour thickness, ulceration of primary lesion, and SLN metastasis, but no significant differences were observed comparing groups of patients with each other, nor when each group of patients was compared with controls (data not shown).

4. Discussion

In the present work, we have investigated for the first time the influence of MICA polymorphism in the extracellular domains on melanoma disease in a group of patients with cutaneous malignant melanoma from southeastern Spain. A total of 17 MICA alleles were detected (see Table 2). MICA allele frequencies found in our control series are similar to those previously reported in other Caucasian populations [22, 36, 37]. Moreover, a number of positive and negative associations with specific MICA alleles were revealed by comparing melanoma patients with healthy individuals. In a first analysis, we found a positive association of MICA^*009 allele with cutaneous malignant melanoma. Immunologically related studies such as those investigating Behçet’s disease also found a positive association between MICA^*009 and disease development [26, 36, 37]. However, in these studies a relationship between HLA-B^*51 and Behçet’s disease was also reported, suggesting that the observed association between MICA^*009 and Behçet’s disease arises from a strong linkage disequilibrium with HLA-B^*51.

Thus, based on the previous linkage disequilibrium reported between MICA^*009 and HLA-B^*51 alleles [22, 32, 33], a further analysis was performed in which we found a high association between MICA^*009 and HLA-B^*51 in both the control and patient groups. This association was stronger in patients than in the control group. Nonetheless, a multivariate logistic regression model showed that neither MICA^*009 nor the combination MICA^*009/HLA-B^*51 was associated with melanoma susceptibility.

Other findings in this study include the decreased frequency of MICA^*002 on melanoma disease. The protective effect of MICA^*002 observed in melanoma disease was also reported in other pathologies like primary sclerosing cholangitis [38]. Nevertheless, studies with larger series are required to find out whether MICA^*002 allele may result in protection from cutaneous melanoma.

On the other hand, no differences were found in the genotype distribution of affinity variants between melanoma patients and controls, unlike what was observed in other pathologic settings [25, 29, 37, 39], where MICA-129 genotype could be considered as a biomarker of disease susceptibility and/or protection.

In accordance with our data, other case-control studies were not able to identify an association between MICA gene polymorphism and the occurrence of melanoma skin cancer, although in this case the polymorphism was studied at transmembrane level [30, 31]. In addition, multiple genome-wide association studies (GWAS) in melanoma patients have identified some genetic variants associated with an increased risk of melanoma development [40–42]. However, these variants are located in several regions (16q24, 11q14-q21, 9p21, 1q21.3, and 20q11.22), other than the region where the MICA gene is placed (6p21.3). All these studies, as well as ours, have failed to establish an association between MICA gene polymorphism and an increased risk of developing cutaneous malignant melanoma.

In conclusion, our data demonstrate a lack of association between MICA polymorphism in the extracellular domains and cutaneous malignant melanoma, at least in our population.

Abbreviations

MHC:	Major histocompatibility complex
MIC:	Major histocompatibility complex class I chain-related gene
MICA:	Major histocompatibility complex class I chain-related gene A
HLA:	Human leukocyte antigen
PCR:	Polymerase chain reaction.

Conflict of Interests

The authors have no conflict of interests to declare.

Authors’ Contribution

The contribution by José Antonio Campillo and Ruth López-Hernández is equal and the order of authorship is arbitrary.

Acknowledgments

The authors wish to thank J. M. Alemany, M. J. Sanchís, and M. C. García-Calatayud for their excellent technical assistance, A. Mrowiec for paper edition and proofreading, and the Immunology, Dermatology and Pathology Services of University Clinical Hospital Virgen de la Arrixaca in Murcia for their kind collaboration. This work was supported by grants from Fondo de Investigación Sanitaria (FIS), Instituto de Salud Carlos III (ISCIII), Ministerio de Sanidad y Consumo (Projects PI-11/02644 and PI-11/02686), grant from Séneca Foundation, Consejería de Educación CCAA Murcia (project 04487/GERM/06), and funding from Caja Murcia. Ruth López-Hernández (CA11/00034) and Lourdes Gimeno (CA09/00099) are postdoctoral researchers supported by FIS, and Fundación para la Formación e Investigación Sanitaria de la Región de Murcia (FFIS), Instituto Murciano de Investigación Sanitaria de la Región de Murcia (IMIB).

References

J. G. Casado, G. Pawelec, S. Morgado et al., “Expression of adhesion molecules and ligands for activating and costimulatory receptors involved in cell-mediated cytotoxicity in a large panel of human melanoma cell lines,” Cancer Immunology, Immunotherapy, vol. 58, no. 9, pp. 1517–1526, 2009.
View at: Publisher Site | Google Scholar
P. P. Lee, C. Yee, P. A. Savage et al., “Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients,” Nature Medicine, vol. 5, no. 6, pp. 677–685, 1999.
View at: Publisher Site | Google Scholar
S. Morgado, B. Sanchez-Correa, J. G. Casado et al., “NK cell recognition and killing of melanoma cells is controlled by multiple activating receptor-ligand interactions,” Journal of Innate Immunity, vol. 3, no. 4, pp. 365–373, 2011.
View at: Publisher Site | Google Scholar
A. Anichini, C. Vegetti, and R. Mortarini, “The paradox of T cell-mediated antitumor immunity in spite of poor clinical outcome in human melanoma,” Cancer Immunology, Immunotherapy, vol. 53, no. 10, pp. 855–864, 2004.
View at: Publisher Site | Google Scholar
V. Groh, J. Wu, C. Yee, and T. Spies, “Tumour-derived soluble MIC ligands impair expression of NKG2D and T-cell activation,” Nature, vol. 419, no. 6908, pp. 734–738, 2002.
View at: Publisher Site | Google Scholar
G. Konjević, K. Mirjačić Martinović, A. Vuletić et al., “Low expression of CD161 and NKG2D activating NK receptor is associated with impaired NK cell cytotoxicity in metastatic melanoma patients,” Clinical and Experimental Metastasis, vol. 24, no. 1, pp. 1–11, 2007.
View at: Publisher Site | Google Scholar
K. M. Martinovic, G. Konjević, N. Babović, and M. Inić, “The stage dependent changes in NK cell activity and the expression of activating and inhibitory NK cell receptors in melanoma patients,” Journal of Surgical Research, vol. 171, no. 2, pp. 637–649, 2011.
View at: Publisher Site | Google Scholar
D. Pende, P. Rivera, S. Marcenaro et al., “Major histocompatibility complex class I-related chain A and UL16-binding protein expression on tumor cell lines of different histotypes: analysis of tumor susceptibility to NKG2D-dependent natural killer cell cytotoxicity,” Cancer Research, vol. 62, no. 21, pp. 6178–6186, 2002.
View at: Google Scholar
L. Moretta, C. Bottino, D. Pende, R. Castriconi, M. C. Mingari, and A. Moretta, “Surface NK receptors and their ligands on tumor cells,” Seminars in Immunology, vol. 18, no. 3, pp. 151–158, 2006.
View at: Publisher Site | Google Scholar
B. Seliger, H. Abken, and S. Ferrone, “HLA-G and MIC expression in tumors and their role in anti-tumor immunity,” Trends in Immunology, vol. 24, no. 2, pp. 82–87, 2003.
View at: Publisher Site | Google Scholar
C. S. Vetter, V. Groh, P. T. Straten, T. Spies, E.-B. Bröcker, and J. C. Becker, “Expression of stress-induced MHC class I related chain molecules on human melanoma,” Journal of Investigative Dermatology, vol. 118, no. 4, pp. 600–605, 2002.
View at: Publisher Site | Google Scholar
P. Carrega, G. Pezzino, P. Queirolo et al., “Susceptibility of human melanoma cells to autologous natural killer (NK) cell killing: HLA-related effector mechanisms and role of unlicensed NK cells,” PLoS ONE, vol. 4, no. 12, Article ID e8132, 2009.
View at: Publisher Site | Google Scholar
D. Pende, C. Cantoni, P. Rivera et al., “Role of NKG2D in tumor cell lysis mediated by human NK cells: cooperation with natural cytotoxicity receptors and capability of recognizing tumors of nonepithelial origin,” European Journal of Immunology, vol. 31, no. 4, pp. 1076–1086, 2001.
View at: Google Scholar
S. Bauer, V. Groh, J. Wu et al., “Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA,” Science, vol. 285, no. 5428, pp. 727–729, 1999.
View at: Publisher Site | Google Scholar
M. Kasahara and S. Yoshida, “Immunogenetics of the NKG2D ligand gene family,” Immunogenetics, vol. 64, no. 12, pp. 855–867, 2012.
View at: Publisher Site | Google Scholar
D. H. Raulet, “Roles of the NKG2D immunoreceptor and its ligands,” Nature Reviews Immunology, vol. 3, no. 10, pp. 781–790, 2003.
View at: Publisher Site | Google Scholar
M. Muro, R. López-Hernández, S. Llorente et al., “MICA molecules in disease and transplantation, a double-edged sword?” Current Immunology Reviews, vol. 8, no. 4, pp. 307–325, 2012.
View at: Publisher Site | Google Scholar
N. W. Zwirner, M. A. Fernández-Viña, and P. Stastny, “MICA, a new polymorphic HLA-related antigen, is expressed mainly by keratinocytes, endothelial cells, and monocytes,” Immunogenetics, vol. 47, no. 2, pp. 139–148, 1998.
View at: Publisher Site | Google Scholar
M. B. Fuertes, L. E. Rossi, C. M. Peralta, H. N. Cabrera, M. A. Allevato, and N. W. Zwirner, “Premalignant quiescent melanocytic nevi do not express the MHC class I chain-related protein A,” Medicina, vol. 71, no. 4, pp. 357–360, 2011.
View at: Google Scholar
S. Bahram, M. Bresnahan, D. E. Geraghty, and T. Spies, “A second lineage of mammalian major histocompatibility complex class I genes,” Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 14, pp. 6259–6263, 1994.
View at: Publisher Site | Google Scholar
N. Mizuki, M. Ota, M. Kimura et al., “Triplet repeat polymorphism in the transmembrane region of the MICA gene: a strong association of six GCT repetitions with Behçet disease,” Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 4, pp. 1298–1303, 1997.
View at: Publisher Site | Google Scholar
E. W. Petersdorf, K. B. Shuler, G. M. Longton, T. Spies, and J. A. Hansen, “Population study of allelic diversity in the human MHC class I-related MIC-A gene,” Immunogenetics, vol. 49, no. 7-8, pp. 605–612, 1999.
View at: Publisher Site | Google Scholar
A. Steinle, P. Li, D. L. Morris et al., “Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family,” Immunogenetics, vol. 53, no. 4, pp. 279–287, 2001.
View at: Publisher Site | Google Scholar
M.-K. Choy and M. E. Phipps, “MICA polymorphism: biology and importance in immunity and disease,” Trends in Molecular Medicine, vol. 16, no. 3, pp. 97–106, 2010.
View at: Publisher Site | Google Scholar
R. López-Hernández, M. Valdés, D. Lucas et al., “Association analysis of MICA gene polymorphism and MICA-129 dimorphism with inflammatory bowel disease susceptibility in a Spanish population,” Human Immunology, vol. 71, no. 5, pp. 512–514, 2010.
View at: Publisher Site | Google Scholar
N. Mizuki, A. Meguro, I. Tohnai, A. Gül, S. Ohno, and N. Mizuki, “Association of major histocompatibility complex class I chain-related gene A and HLA-B alleles with Behçet's disease in Turkey,” Japanese Journal of Ophthalmology, vol. 51, no. 6, pp. 431–436, 2007.
View at: Publisher Site | Google Scholar
G. García, F. Del Puerto, A. B. Pérez et al., “Association of MICA and MICB alleles with symptomatic dengue infection,” Human Immunology, vol. 72, no. 10, pp. 904–907, 2011.
View at: Publisher Site | Google Scholar
S. Vallian, M. J. Rad, M. Tavallaei, and M. Tavassoli, “Correlation of major histocompatibility complex class I related A (MICA) polymorphism with the risk of developing breast cancer,” Medical Oncology, vol. 29, no. 1, pp. 5–9, 2012.
View at: Publisher Site | Google Scholar
W. Boukouaci, M. Busson, R. P. de Latour et al., “MICA-129 genotype, soluble MICA, and anti-MICA antibodies as biomarkers of chronic graft-versus-host disease,” Blood, vol. 114, no. 25, pp. 5216–5224, 2009.
View at: Publisher Site | Google Scholar
C. Kennedy, A. Naipal, N. A. Gruis et al., “MICA gene polymorphism is not associated with an increased risk for skin cancer,” Journal of Investigative Dermatology, vol. 118, no. 4, pp. 686–691, 2002.
View at: Publisher Site | Google Scholar
J. A. W. Metzelaar-Blok, H. M. H. Hurks, A. Naipal et al., “Normal HLA class I, II, and MICA gene distribution in uveal melanoma,” Molecular Vision, vol. 11, pp. 1166–1172, 2005.
View at: Google Scholar
X. Gao, R. M. Single, P. Karacki, D. Marti, S. J. O'Brien, and M. Carrington, “Diversity of MICA and linkage disequilibrium with HLA-B in two north American populations,” Human Immunology, vol. 67, no. 3, pp. 152–158, 2006.
View at: Publisher Site | Google Scholar
D. Lucas, J. A. Campillo, R. López-Hernández et al., “Allelic diversity of MICA gene and MICA/HLA-B haplotypic variation in a population of the Murcia region in southeastern Spain,” Human Immunology, vol. 69, no. 10, pp. 655–660, 2008.
View at: Publisher Site | Google Scholar
C. M. Balch, J. E. Gershenwald, S.-J. Soong et al., “Final version of 2009 AJCC melanoma staging and classification,” Journal of Clinical Oncology, vol. 27, no. 36, pp. 6199–6206, 2009.
View at: Publisher Site | Google Scholar
A. Svejgaard and L. P. Ryder, “HLA and disease associations: detecting the strongest association,” Tissue Antigens, vol. 43, no. 1, pp. 18–27, 1994.
View at: Publisher Site | Google Scholar
E. H. Hughes, R. W. M. Collins, E. Kondeatis et al., “Associations of major histocompatibility complex class I chain-related molecule polymorphisms with Behcet's disease in Caucasian patients,” Tissue Antigens, vol. 66, no. 3, pp. 195–199, 2005.
View at: Publisher Site | Google Scholar
I. Muñoz-Saá, A. Cambra, L. Pallarés et al., “Allelic diversity and affinity variants of MICA are imbalanced in Spanish patients with Behçet's disease,” Scandinavian Journal of Immunology, vol. 64, no. 1, pp. 77–82, 2006.
View at: Publisher Site | Google Scholar
S. Norris, E. Kondeatis, R. Collins et al., “Mapping MHC-encoded susceptibility and resistance in primary sclerosing cholangitis: the role of MICA polymorphism,” Gastroenterology, vol. 120, no. 6, pp. 1475–1482, 2001.
View at: Publisher Site | Google Scholar
H. Douik, A. B. Chaaben, N. A. Romdhane et al., “Association of MICA-129 polymorphism with nasopharyngeal cancer risk in a Tunisian population,” Human Immunology, vol. 70, no. 1, pp. 45–48, 2009.
View at: Publisher Site | Google Scholar
D. T. Bishop, F. Demenais, M. M. Iles et al., “Genome-wide association study identifies three loci associated with melanoma risk,” Nature Genetics, vol. 41, no. 8, pp. 920–925, 2009.
View at: Publisher Site | Google Scholar
S. MacGregor, G. W. Montgomery, J. Z. Liu et al., “Genome-wide association study identifies a new melanoma susceptibility locus at 1q21.3,” Nature Genetics, vol. 43, no. 11, pp. 1114–1118, 2011.
View at: Publisher Site | Google Scholar
K. M. Brown, S. MacGregor, G. W. Montgomery et al., “Common sequence variants on 20q11.22 confer melanoma susceptibility,” Nature Genetics, vol. 40, no. 7, pp. 838–840, 2008.
View at: Publisher Site | Google Scholar

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Copyright © 2015 José Antonio Campillo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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