miR-34a Inhibits Proliferation and Invasion of Bladder Cancer Cells by Targeting Orphan Nuclear Receptor HNF4G

Figure 2

miR-34a inhibits cell viability, colony formation rate, and invasion of bladder cancer cells (a) MTT assay of cell viability. Knockdown (a1) and overexpression (a2) of miR-34a caused significantly promotion and inhibition of cell viability, respectively, in a time dependent manner. (b) Colony formation assay. Knockdown of miR-34a caused 37.3% increase of colony formation rate in 5637-NM cells (b1), while overexpressing miR-34a reduced it by 32.6% in muscle invasive counterpart (b2). (c) Transwell assay of cell invasion. The cell invasion assay indicated that miR-34a knockdown in 5637-NM cells increased cell invasion (c1) while overexpressing miR-34a in 5637-M cells inhibited cell invasion (c2). . Error bars depict s.e.m.