miR-34a Inhibits Proliferation and Invasion of Bladder Cancer Cells by Targeting Orphan Nuclear Receptor HNF4G

Figure 4

miR-34a inhibits HNF4G expression to reduce bladder cancer proliferation and invasion. (a) Western blot analysis of HNF4G expression in 5637-NM (a1) and 5636-M (a2) cells with HNF4G overexpression and knockdown, respectively. (b) MTT assay showed that overexpressing HNF4G in 5637-NM (b1) and knockdown in 5637-M (b2) cells promoted and suppressed cell growth, respectively. (c) HNF4G promoted colony formation. Overexpressing HNF4G caused 67.8% increase of colony formation rate in 5637-NM cells (c1), while inhibiting its expression reduced the rate by 49.4% in invasive counterpart (c2). (d) The cell invasion assay indicated that knockdown of HNF4G in 5637-M cells inhibited cell invasion while overexpressing it enhanced cell invasion. miR-34a significantly abrogated HNF4G-wt induced higher cell invasion but had no effect on HNF4G-mut induced cell invasion increase. , . Error bars depict s.e.m.