The Analysis of Sialylation, N-Glycan Branching, and Expression of O-Glycans in Seminal Plasma of Infertile Men

Table 1

Relative reactivity of human seminal plasma glycoproteins with lectins.

Groups	Relative reactivity with lectins (AU)
	MAA	SNA	MPL	VVL	PHA-L
Normozoospermic fertile	0.409 ± 0.120	0.505 ± 0.125	1.030 ± 0.193	0.477 ± 0.225	0.424 ± 0.172
Normozoospermic infertile	0.488 ± 0.159	0.485 ± 0.145	0.879 ± 0.150; = 0.0013	0.520 ± 0.284	0.363 ± 0.167
Asthenozoospermic	0.501 ± 0.145	0.380 ± 0.127; = 0.012	0.968 ± 0.196	0.497 ± 0.292	0.337 ± 0.169
Oligozoospermic	0.297 ± 0.097; = 0.00015	0.509 ± 0.107; = 0.014	0.822 ± 0.085; = 0.0014	0.423 ± 0.130	0.211 ± 0.110; = 0.00027; = 0.01
Oligoasthenozoospermic	0.409 ± 0.133	0.407 ± 0.117	0.944 ± 0.226	0.557 ± 0.255	0.313 ± 0.136

The results are expressed in absorbance units (AU) as mean values ± SD. Seminal glycoproteins’ lectin reactivities were estimated by direct lectin-ELISA and expressed in absorbance units (AU). MAA: Maackia amurensis agglutinin, SNA: Sambucus nigra agglutinin, MPL: Maclura pomifera lectin, VVL: Vicia villosa lectin, and PHA-L: Phaseolus vulgaris leucoagglutinin. For lectin specificity, see Section 2. Significant differences versus ¹normozoospermic fertile, ²normozoospermic infertile, and ³asthenozoospermic groups. Statistical significance of the differences was accepted for a value of less than 0.05.