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Disease Markers
Volume 2016 (2016), Article ID 4703854, 6 pages
http://dx.doi.org/10.1155/2016/4703854
Research Article

RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population

1Institute of Tropical Medicine and Infectious Diseases, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya
2Institute of Biotechnology, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya
3Department of Plant and Microbial Sciences, Kenyatta University, Nairobi, Kenya
4Center for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya

Received 21 April 2016; Revised 5 August 2016; Accepted 9 August 2016

Academic Editor: Giuseppe Murdaca

Copyright © 2016 Shem P. M. Mutuiri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Previous studies have reported that two single nucleotide polymorphisms (SNPs) in the RANTES gene promoter region, -403G/A and -28C/G, are associated with a slower rate of decline in CD4+ T cell count. In addition, as a ligand of the major HIV coreceptor CCR5, it is known to block HIV-CCR5 interactions in the course of the HIV infection cycle. This study was carried out with the aim of determining the occurrence of single nucleotide polymorphisms (SNPs) -403G > A and -28C > G in the promoter region of RANTES, in a subset of the Kenyan population. Genomic DNA was extracted from peripheral blood monocular cells and used to amplify the RANTES gene region. Restriction fragment length polymorphism was used to determine the genotypes of the RANTES gene. Out of 100 HIV infected individuals, 19% had G1 genotypes (403G/G, 28C/G), 30% (403A/A, 28C/C), and 50% (403G/A, 28C/C), while in healthy blood donors 13% had G4 (403G/A, 28C/C) genotypes, 22% (403A/A, 28C/C), and 54% (403G/A, 28C/C). HIV negative blood donors (54%) had higher risk of alteration to risk of HIV transmission compared to those who were HIV infected (50%). However, the risk to transmission and distribution differences was not significant (). The study showed that RANTES polymorphisms -403 and -28 alleles do exist in the Kenyan population.