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Disease Markers
Volume 2016, Article ID 9172726, 5 pages
http://dx.doi.org/10.1155/2016/9172726
Research Article

Influence of βS-Globin Haplotypes and Hydroxyurea on Arginase I Levels in Sickle Cell Disease

1Post-Graduate Program in Pathology, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-370 Fortaleza, CE, Brazil
2Post-Graduate Program in Development and Technological Innovation in Drugs, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-370 Fortaleza, CE, Brazil
3Department of Clinical and Toxicological Analysis, University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-370 Fortaleza, CE, Brazil
4Post-Graduate Program in Pharmaceutical Sciences, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-370 Fortaleza, CE, Brazil

Received 22 October 2015; Revised 17 February 2016; Accepted 24 February 2016

Academic Editor: Irene Rebelo

Copyright © 2016 J. A. Moreira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. Sickle cell disease (SCD) is characterized by hemoglobin S homozygosity, leading to hemolysis and vasoocclusion. The hemolysis releases arginase I, an enzyme that decreases the bioavailability of nitric oxide, worsening the symptoms. The different SCD haplotypes are related to clinical symptoms and varied hemoglobin F (HbF) concentration. The aim of this study was to evaluate the impact of the βS gene haplotypes and HbF concentration on arginase I levels in SCD patients. Methods. Fifty SCD adult patients were enrolled in the study and 20 blood donors composed the control group. Arginase I was measured by ELISA. The βS haplotypes were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Statistical analyses were performed with GraphPad Prism program and the significance level was . Results. Significant increase was observed in the arginase I levels in SCD patients compared to the control group (). The comparison between the levels of arginase I in three haplotypes groups showed a difference between the Bantu/Bantu Bantu/Benin groups; Bantu/Bantu Benin/Benin, independent of HU dosage. An inverse correlation with the arginase I levels and HbF concentration was observed. Conclusion. The results support the hypothesis that arginase I is associated with HbF concentration, also measured indirectly by the association with haplotypes.