SFRP Tumour Suppressor Genes Are Potential Plasma-Based Epigenetic Biomarkers for Malignant Pleural Mesothelioma

Figure 1

Asbestos-induced downregulation of tumour suppressor gene expression due to DNA methylation. (a) Basal expression and demethylated expression of mRNA of SFRP genes were determined by RT-qPCR in 7 MPM cell lines and in MeT-5A. Results were normalized to 18S and are expressed relative to the expression of MeT-5A or control untreated cells. (b) The methylation status of SFRP2 and SFRP5 were determined by MSP cell lines. (c) The expression of SFRP1 was determined by RT-qPCR and DNA methylation by qMSP in MeT-5A cells with or without asbestos exposure. mRNA expression or methylation status was presented as fold change to parental untreated MeT-5A cells. (M = methylated; U = unmethylated, BC = buffy coat isolated from a healthy donor, IVD = universal methylated DNA control).

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