Research Article

SFRP Tumour Suppressor Genes Are Potential Plasma-Based Epigenetic Biomarkers for Malignant Pleural Mesothelioma

Figure 3

SFRP2 or SFRP5 re-expression in MPM cell lines inhibits cell growth and colony formation. (a) Protein re-expression of SFRP2 and SFRP5 was confirmed with immunofluorescence SFRP2 or SFRP5 red or β-actin in green and DAPI nuclear staining in blue. (b) 11 MPM cell lines and MeT-5A were transfected with pcDNA3.1 or pcDNA3.1-SFRP2 or pcDNA3.1-SFRP5, and plates were harvested every 24 hrs for a total of 120 hrs. Cell proliferation was determined by SYBG assay, significant difference between SFRP2 and SFRP5 to control as with . (c) The clonogenic potential was assessed by plating 2500 transfected cells per 96 wells and then transferring to a 6-well plate at 24 hrs posttransfection, then incubated for a further 10–14 days. A representative picture from three independent experiments is shown.
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