Table of Contents Author Guidelines Submit a Manuscript
Disease Markers
Volume 2017, Article ID 4862016, 11 pages
Research Article

Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms

1Faculty of Chemistry, Autonomous University of the State of Mexico (UAEMex), Toluca, MEX, Mexico
2Specialized Hemato-Oncological Laboratory of the Children’s Hospital, IMIEM, Toluca, MEX, Mexico
3Molecular Biology Laboratory, Medical Research Center (CICMED), Toluca, MEX, Mexico
4Laboratory of Immunohistochemistry, National Pediatrics Institute, Coyoacán, MEX, Mexico

Correspondence should be addressed to Jonnathan Guadalupe Santillán-Benítez; xm.moc.oohay@bsgnahtannoj

Received 24 May 2017; Accepted 20 July 2017; Published 5 September 2017

Academic Editor: Stefano M. M. Basso

Copyright © 2017 Martha Daniela Mociño-Rodríguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The development of breast cancer is influenced by the adipose tissue through the proteins leptin and adiponectin. However, there is little research concerning AdipoR1 and AdipoR2 receptors and the influence of leptin over them. The objective of this work was to analyze the expression of AdipoR1 and AdipoR2, modulated by differential concentrations of leptin in an obesity model (10 ng/mL, 100 ng/mL, and 1000 ng/mL) associated with breast cancer in MCF-7 and HCC1937 cell lines. Each cell line was characterized through immunohistochemistry, and the expression of AdipoR1 and AdipoR2 was analyzed by PCR in real time using TaqMan® probes. Leptin induced an increase in cell population of MCF-7 (23.8%, 10 ng/mL, 48 h) and HCC1937 (17.24%, 1000 ng/mL, 72 h). In MCF-7, the expression of AdipoR1 decreased (3.81%, 1000 ng/mL) and the expression of AdipoR2 increased by 13.74 times (10 ng/mL) with regard to the control. In HCC1937, the expression of AdipoR1 decreased by 86.28% (10 ng/mL), as well as the expression of AdipoR2 (50.3%, 100 ng/mL). In regard to the results obtained, it could be concluded that leptin has an effect over the expression of AdipoR1 and AdipoR2 mRNA.