LncRNA NEAT1 Regulates Cell Viability and Invasion in Esophageal Squamous Cell Carcinoma through the miR-129/CTBP2 Axis

Figure 4

NEAT1 regulated CTBP2 expression by competitively binding to miR-129. (a) Putative binding regions between CTBP2 and miR-129. The relative luciferase activity of EC109 (b) and EC9706 (c) cells cotransfected with pMIR luciferase reporter vectors containing wild-type or mutated CTBP2 3′UTR and miR-129 or miR-control. (d) Western blot analysis of CTBP2 expression in EC109 and EC9706 cells transfected with miR-129 or miR-control. The relative luciferase activity of EC109 (e) and EC9706 (f) cells cotransfected with CTBP2-WT, CTBP2-WT + miR-129, or in combination with pcDNA or pcDNA-NEAT1. The protein level of CTBP2 in EC109 (g) and EC9706 (h) cells transfected with si-NEAT1, si-control, si-NEAT1 + anti-miR-control, or si-NEAT1 + anti-miR-129. .

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