miR-135b Plays a Neuroprotective Role by Targeting GSK3β in MPP+-Intoxicated SH-SY5Y Cells
Effect of miR-135b overexpression on viability, apoptosis, and neuroinflammation in MPP+-intoxicated SH-SY5Y cells. SH-SY5Y cells were transfected with miR-135b or miR-NC prior to 1 mM MPP+ treatment for 24 h. (a) MTT assay was used to determine cell viability in treated SH-SY5Y cells. (b) Apoptosis of treated SH-SY5Y cells was detected by TUNEL assay. (c) Quantification of TUNEL staining was shown. (d) Western blot was performed to evaluate the level of cleaved caspase 3 in treated SH-SY5Y cells. (e) Quantitative analysis of cleaved caspase 3 level. β-actin was used as the normalization. The concentrations of IL-1β (f) and TNF-α (g) in treated SH-SY5Y cells were detected by ELISA. versus control group.