miR-135b Plays a Neuroprotective Role by Targeting GSK3β in MPP⁺-Intoxicated SH-SY5Y Cells

Figure 5

Activation of GSK3β reversed the effects of miR-135b on proliferation, apoptosis, and neuroinflammation in MPP⁺-intoxicated SH-SY5Y cells. SH-SY5Y cells with miR-135b transfection were cocultured with 2 mM SNP for 48 h followed by exposing to 1 mM MPP⁺ for 24 h. (a) Cell viability was determined by MTT assay in treated SH-SY5Y cells. (b) Apoptosis of treated SH-SY5Y cells was assessed by TUNEL assay. (c and d) Western blot was carried out to detect the level of cleaved caspase 3 in treated SH-SY5Y cells. The concentrations of TNF-α (e) and IL-1β (f) in treated SH-SY5Y cells were measured by ELISA. versus control group.

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