Genotyping of IL-4 −590 (C>T) Gene in Iraqi Asthma Patients

Table 2

PCR mix reaction for genotyping of IL-4 gene position −590 (C>T).

Component	Volume (μl)	Final concentration
Each primer (T or C allele + reverse)	2	1 μM
DNA template	5	100 ng
Nuclease-free water	11	—
Final volume	20	—

T and reverse primers were used for detecting T allele; C and reverse primers were used for detecting C allele.