Research Article

Role of MMP-1 (-519A/G, -1607 1G/2G), MMP-3 (Lys45Glu), MMP-7 (-181A/G), and MMP-12 (-82A/G) Variants and Plasma MMP Levels on Obesity-Related Phenotypes and Microvascular Reactivity in a Tunisian Population

Figure 1

Genotyping of MMP-1 (-519A/G; -1607 1G/2G), MMP-3 Lys45Glu (A/G), MMP-7 (-181A/G), and MMP-12 (-82A/G) by PCR-RFLP. (a) MMP-1 (-519A/G): the product (200 bp) was digested with KpnI. The A allele corresponds to the 176 bp and 24 bp fragments. The G allele corresponds to the 200 bp. (b) MMP-1 (-1607 1G/2G): the PCR product (118 bp) was digested with XmnI. The 1G allele corresponds to the 79 bp, 28 bp, and 11 bp. The 2G allele corresponds to the 107 bp and 11 bp fragments. (c) MMP-3 Lys45Glu (A/G): the PCR product (748 bp) was digested with TaqI. The G allele corresponds to the 432 bp and 316 bp fragments. The A allele corresponds to the 748 bp fragment. (d) MMP-7 (-181A/G): the PCR product (150 bp) was digested with EcoRI. The A allele corresponds to the 150 bp fragment. The G allele corresponds to the120 bp and 30 bp fragments. (e) MMP-12 (-82A/G): the PCR product (199 bp) was digested with PvuII. The A allele corresponds to the 199 bp fragment. The G allele corresponds to the 175 bp and 24 bp fragments.
(a) MMP-1 (-519A/G)
(b) MMP-1 (-16071G/2G)
(c) MMP-3 Lys45Glu (A/G)
(d) MMP-7 (-181A/G)
(e) MMP-12 (-82A/G)