Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation

Figure 6

Leptin-induced EmCa cell invasion was abrogated by inhibition of Notch and IL-1 signaling. (a) Quantitative assessment of Ishikawa (derived from type I EmCa) cell invasion. (b) Representative pictures of the effects of leptin and NILCO inhibitors on Ishikawa cell invasion. (c) Quantitative assessment of An3ca (derived from type II EmCa) cell invasion. (d) Representative pictures of the effects of leptin and NILCO inhibitors on An3ca cell invasion. Results from cell migration (Boyden chamber cell invasion assay; Corning BioCoat Matrigel invasion chamber) were obtained after 24 h and normalized to basal conditions. Invading cells were detected by hematoxylin staining (see Section 2). Data (mean ± standard error) representative results derived from a minimum of 3 independent experiments. .

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