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Disease Markers
Volume 2017, Article ID 9645940, 10 pages
https://doi.org/10.1155/2017/9645940
Research Article

MicroRNA Expression in Malignant Pleural Mesothelioma and Asbestosis: A Pilot Study

1Molecular Genetics, Department of Medicine and Surgery, University of Parma, Parma, Italy
2Thoracic Surgery, Department of Medicine and Surgery, University of Parma, Parma, Italy
3Department of Medicine and Surgery, University of Parma, Parma, Italy
4Medical Oncology, University Hospital of Parma, Parma, Italy
5Pathological Anatomy and Histology, University Hospital of Parma, Parma, Italy

Correspondence should be addressed to Matteo Goldoni; ti.rpinu@inodlog.oettam

Received 21 February 2017; Revised 21 May 2017; Accepted 5 June 2017; Published 3 July 2017

Academic Editor: Alvaro González

Copyright © 2017 Paola Mozzoni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. W. Zhang, X. Wu, L. Wu, W. Zhang, and X. Zhao, “Advances in the diagnosis, treatment and prognosis of malignant pleural mesothelioma,” Annals of translational medicine, vol. 3, no. 13, p. 182, 2015. View at Publisher · View at Google Scholar
  2. C. Norbet, A. Joseph, S. S. Rossi, S. Bhalla, and F. R. Gutierrez, “Asbestos-related lung disease: a pictorial review,” Current Problems in Diagnostic Radiology, vol. 44, no. 4, pp. 371–382, 2015. View at Publisher · View at Google Scholar · View at Scopus
  3. B. I. Hiddinga, C. Rolfo, and J. P. van Meerbeeck, “Mesothelioma treatment: are we on target? A review,” Journal of Advanced Research, vol. 6, no. 3, pp. 319–330, 2015. View at Publisher · View at Google Scholar · View at Scopus
  4. A. Osman, “MicroRNAs in health and disease-basic science and clinical applications,” Clinical Laboratory, vol. 58, no. 5-6, pp. 393–402, 2012. View at Google Scholar
  5. T. Treiber, N. Treiber, and G. Meister, “Regulation of microRNA biogenesis and function,” Thrombosis and Haemostasis, vol. 107, no. 4, pp. 605–610, 2012. View at Publisher · View at Google Scholar · View at Scopus
  6. E. Lages, H. Ipas, A. Guttin, H. Nesr, F. Berger, and J. P. Issartel, “MicroRNAs: molecular features and role in cancer,” Frontiers in Bioscience, vol. 17, pp. 2508–2540, 2012. View at Publisher · View at Google Scholar · View at Scopus
  7. S. I. Rothschild, “Epigenetic therapy in lung cancer. Role of microRNAs,” Frontiers in Oncology, vol. 3, p. 158, 2013. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Boeri, U. Pastorino, and G. Sozzi, “Role of microRNAs in lung cancer: microRNA signatures in cancer prognosis,” Cancer Journal, vol. 18, no. 3, pp. 268–274, 2012. View at Publisher · View at Google Scholar · View at Scopus
  9. A. Esquela-Kerscher and F. J. Slack, “Oncomirs-microRNAs with a role in cancer,” Nature Reviews. Cancer, vol. 6, no. 4, pp. 259–269, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. U. Vosa, T. Vooder, R. Kolde, J. Vilo, A. Metspalu, and T. Annilo, “Meta-analysis of microRNA expression in lung cancer,” International Journal of Cancer, vol. 132, no. 12, pp. 2884–2893, 2013. View at Publisher · View at Google Scholar · View at Scopus
  11. G. A. Calin, A. Cimmino, and M. Fabbri, “MiR-15a and miR-16-1 cluster functions in human leukemia,” Proceedings of the National Academy of Sciences USA, vol. 105, no. 13, pp. 5166–5171, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. R. I. Aqeilan, G. A. Calin, and C. M. Croce, “miR-15a and miR-16-1 in cancer: discovery, function and future perspectives,” Cell Death and Differentiation, vol. 17, no. 2, pp. 215–220, 2010. View at Google Scholar
  13. M. A. Rivas, L. Venturutti, Y. W. Huang, R. Schillaci, T. H. Huang, and P. V. Elizalde, “Downregulation of the tumor-suppressor miR-16 via progestin-mediated oncogenic signaling contributes to breast cancer development,” Breast Cancer Research, vol. 14, no. 3, p. R77, 2012. View at Publisher · View at Google Scholar · View at Scopus
  14. Y. Ke, W. Zhao, J. Xiong, and R. Cao, “Downregulation of miR-16 promotes growth and motility by targeting HDGF in non-small cell lung cancer cells,” FEBS Letters, vol. 587, no. 18, p. 3153, 2013. View at Publisher · View at Google Scholar · View at Scopus
  15. W. Kang, J. H. Tong, R. W. Lung et al., “Targeting of YAP1 by microRNA-15a and microRNA-16-1 exerts tumor suppressor function in gastric adenocarcinoma,” Molecular Cancer, vol. 14, p. 52, 2015. View at Publisher · View at Google Scholar · View at Scopus
  16. N. Bandi, S. Zbinden, M. Gugger et al., “miR-15a and miR-16 are implicated in cell cycle regulation in a Rb-dependent manner and are frequently deleted or down-regulated in non-small cell lung cancer,” Cancer Research, vol. 69, no. 13, pp. 5553–5559, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. C. K. Cai, G. Y. Zhao, L. Y. Tian et al., “miR-15a and miR-16-1 downregulate CCND1 and induce apoptosis and cell cycle arrest in osteosarcoma,” Oncology Reports, vol. 28, no. 5, pp. 1764–1770, 2012. View at Publisher · View at Google Scholar · View at Scopus
  18. L. Y. Liu, W. Wang, L. Y. Zhao et al., “Mir-126 inhibits growth of SGC-7901 cells by synergistically targeting the oncogenes PI3KR2 and Crk, and the tumor suppressor PLK2,” International Journal of Oncology, vol. 45, no. 3, pp. 1257–1265, 2014. View at Publisher · View at Google Scholar · View at Scopus
  19. M. Crawford, E. Brawner, K. Batte et al., “MicroRNA-126 inhibits invasion in non-small cell lung carcinoma cell lines,” Biochemical and Biophysical Research Communications, vol. 373, no. 4, pp. 607–612, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. Y. Qian, X. Wang, Z. Lv et al., “MicroRNA 126 is downregulated in thyroid cancer cells, and regulates proliferation, migration and invasion by targeting CXCR4,” Molecular Medicine Reports, vol. 14, no. 1, pp. 453–459, 2016. View at Publisher · View at Google Scholar · View at Scopus
  21. L. Jiang, A. He, Q. Zhang, and C. Tao, “MiR-126 inhibits cell growth, invasion and migration of osteosarcoma cells by downregulating ADAM-9,” Tumour Biology, vol. 35, pp. 12645–12654, 2014. View at Google Scholar
  22. X. Wang, S. Tang, S. Y. Le et al., “Aberrant expression of oncogenic and tumor-suppressive microRNAs in cervical cancer is required for cancer cell growth,” PLoS One, vol. 3, no. 7, article e2557, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. Y. Liu, Y. Zhou, X. Feng et al., “MicroRNA-126 functions as a tumor suppressor in colorectal cancer cells by targeting CXCR4 via the AKT and ERK1/2 signaling pathways,” International Journal of Oncology, vol. 44, pp. 203–210, 2014. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Salajegheh, H. Vosgha, M. A. Rahman, M. Amin, R. A. Smith, and A. K. Lam, “Interactive role of miR-126 on VEGF-A and progression of papillary and undifferentiated thyroid carcinoma,” Human Pathology, vol. 51, pp. 75–85, 2016. View at Publisher · View at Google Scholar · View at Scopus
  25. R. Tang, L. Pei, T. Bai, and J. Wang, “Down-regulation of microRNA-126-5p contributes to overexpression of VEGFA in lipopolysaccharide-induced acute lung injury,” Biotechnology Letters, vol. 38, no. 8, pp. 1277–1284, 2016. View at Publisher · View at Google Scholar · View at Scopus
  26. H. Chen, L. Li, S. Wang et al., “Reduced miR-126expression facilitates angiogenesis of gastric cancer through its regulation on VEGF-A,” Oncotarget, vol. 5, no. 23, pp. 11873–11885, 2014. View at Publisher · View at Google Scholar
  27. P. Mozzoni, I. Banda, M. Goldoni et al., “Plasma and EBC microRNAs as early biomarkers of non-small-cell lung cancer,” Biomarkers, vol. 18, no. 8, pp. 679–686, 2013. View at Publisher · View at Google Scholar · View at Scopus
  28. J. Zhu, Y. Zeng, C. Xu et al., “Expression profile analysis of microRNAs and downregulated miR-486-5p and miR-30a-5p in non-small cell lung cancer,” Oncology Reports, vol. 34, no. 4, pp. 1779–1786, 2015. View at Publisher · View at Google Scholar · View at Scopus
  29. H. Cai, Y. Yuan, Y. F. Hao, T. K. Guo, X. Wei, and Y. M. Zhang, “Plasma microRNAs serve as novel potential biomarkers for early detection of gastric cancer,” Medical Oncology, vol. 30, no. 1, p. 452, 2013. View at Publisher · View at Google Scholar · View at Scopus
  30. N. Mosakhani, V. K. Sarhadi, I. Borze et al., “MicroRNA profiling differentiates colorectal cancer according to KRAS status,” Genes, Chromosomes & Cancer, vol. 51, no. 1, pp. 1–9, 2012. View at Publisher · View at Google Scholar · View at Scopus
  31. E. Chan, R. Patel, S. Nallur et al., “MicroRNA signatures differentiate melanoma subtypes,” Cell Cycle, vol. 10, no. 11, pp. 1845–1852, 2011. View at Publisher · View at Google Scholar · View at Scopus
  32. R. Ottman, J. Levy, W. E. Grizzle, and R. Chakrabarti, “The other face of miR-17-92 cluster, exhibiting tumor suppressor effects in prostate cancer,” Oncotarget, vol. 7, no. 45, pp. 73739–73753, 2016. View at Publisher · View at Google Scholar · View at Scopus
  33. F. Yang, Y. Li, L. Xu et al., “miR-17 as a diagnostic biomarker regulates cell proliferation in breast cancer,” OncoTargets and Therapy, vol. 10, pp. 543–550, 2017. View at Publisher · View at Google Scholar
  34. C. Chen, Z. Lu, J. Yang et al., “MiR-17-5p promotes cancer cell proliferation and tumorigenesis in nasopharyngeal carcinoma by targeting p21,” Cancer Medicine, vol. 5, no. 12, pp. 3489–3499, 2016. View at Publisher · View at Google Scholar · View at Scopus
  35. V. Balatti, S. Maniero, M. Ferracin et al., “MicroRNAs dysregulation in human malignant pleural mesothelioma,” Journal of Thoracic Oncology, vol. 6, no. 5, pp. 844–851, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. S. Busacca, S. Germano, L. De Cecco et al., “MicroRNA signature of malignant mesothelioma with potential diagnostic and prognostic implications,” American Journal of Respiratory Cell and Molecular Biology, vol. 42, no. 3, pp. 312–319, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. M. Andersen, M. Grauslund, M. Muhammad-Ali et al., “Are differentially expressed microRNAs useful in the diagnostics of malignant pleural mesothelioma?” APMIS, vol. 120, no. 9, pp. 767–769, 2012. View at Publisher · View at Google Scholar · View at Scopus
  38. R. Ottman, C. Nguyen, R. Lorch, and R. Chakrabarti, “MicroRNA expressions associated with progression of prostate cancer cells to antiandrogen therapy resistance,” Molecular Cancer, vol. 13, p. 1, 2014. View at Publisher · View at Google Scholar · View at Scopus
  39. W. Zhang, J. Lin, P. Wang, and J. Sun, “miR-17-5p down-regulation contributes to erlotinib resistance in non-small cell lung cancer cells,” Journal of Drug Targeting, vol. 25, no. 2, pp. 125–131, 2017. View at Publisher · View at Google Scholar · View at Scopus
  40. L. Wong, K. Lee, I. Russell, and C. Chen, “Endogenous controls for real-time quantitation of miRNA using TaqMan® MicroRNA assays,” in Applied Biosystems Application Note, pp. P11–P01, Applied Biosystems, CA, USA. View at Publisher · View at Google Scholar
  41. J. Rice, H. Roberts, S. N. Rai, and S. Galandiuk, “Housekeeping genes for studies of plasma microRNA: a need for more precise standardization,” Surgery, vol. 158, no. 5, pp. 1345–1351, 2015. View at Publisher · View at Google Scholar · View at Scopus
  42. S. Papaspyros and S. Papaspyros, “Surgical management of malignant pleural mesothelioma: impact of surgery on survival and quality of life-relation to chemotherapy, radiotherapy, and alternative therapies,” ISRN Surgery, vol. 3, p. 2014, 2014. View at Publisher · View at Google Scholar
  43. S. Prazakova, P. S. Thomas, A. Sandrini, and D. H. Yates, “Asbestos and the lung in the 21st century: an update,” The Clinical Respiratory Journal, vol. 8, no. 1, pp. 1–10, 2014. View at Publisher · View at Google Scholar · View at Scopus
  44. G. Liu, P. Cheresh, and D. W. Kamp, “Molecular basis of asbestos-induced lung disease,” Annual Review of Pathology, vol. 8, pp. 161–187, 2013. View at Publisher · View at Google Scholar · View at Scopus
  45. A. Markou, L. Sourvinou, P. A. Vorkas, G. M. Yousef, and E. Lianidou, “Clinical evaluation of microRNA expression profiling in non small cell lung cancer,” Lung Cancer, vol. 81, no. 3, pp. 388–396, 2013. View at Publisher · View at Google Scholar · View at Scopus
  46. Y. Tang, W. Yan, J. Chen, C. Luo, A. Kaipa, and B. Shen, “Identification of novel microRNA regulatory pathways associated with heterogeneous prostate cancer,” BMC Systems Biology, vol. 7, Supplement 3, p. S6, 2013. View at Publisher · View at Google Scholar · View at Scopus
  47. G. Sozzi, U. Pastorino, and C. M. Croce, “MicroRNAs and lung cancer: from markers to targets,” Cell Cycle, vol. 10, no. 13, pp. 20145-20146, 2001. View at Publisher · View at Google Scholar · View at Scopus
  48. T. Cavalleri, L. Angelici, C. Favero et al., “Plasmaticextracellular vesicle microRNAs in malignant pleural mesothelioma andasbestos-exposed subjects suggest a 2-miRNA signature as potential biomarker ofdisease,” PloS One, vol. 12, no. 5, article e0176680, 2017. View at Publisher · View at Google Scholar
  49. K. A. O'Donnell, E. A. Wentzel, K. I. Zeller, C. V. Dang, and J. T. Mendell, “c-Myc-regulated microRNAs modulate E2F1 expression,” Nature, vol. 435, no. 7043, pp. 839–843, 2005. View at Publisher · View at Google Scholar · View at Scopus
  50. Y. Hayashita, H. Osada, Y. Tatematsu et al., “A polycistronic microRNA cluster, miR-17-92,is overexpressed in human lung cancers and enhances cell proliferation,” CancerResearch, vol. 65, no. 21, pp. 9628–9632, 2005. View at Publisher · View at Google Scholar · View at Scopus
  51. L. He, J. M. Thomson, M. T. Hemann et al., “A microRNA polycistron as a potential human oncogene,” Nature, vol. 435, no. 7043, pp. 828–833, 2005. View at Publisher · View at Google Scholar · View at Scopus
  52. Z. Yu, C. Wang, M. Wang et al., “A cyclin D1/microRNA 17/20 regulatory feedback loop in control of breast cancer cell proliferation,” The Journal of Cell Biology, vol. 182, no. 3, pp. 509–517, 2008. View at Publisher · View at Google Scholar · View at Scopus
  53. V. Panou, M. Vyberg, U. M. Weinreich, C. Meristoudis, U. G. Falkmer, and O. D. Røe, “The established and future biomarkers of malignant pleural mesothelioma,” Cancer Treatment Reviews, vol. 41, no. 6, pp. 486–495, 2015. View at Publisher · View at Google Scholar · View at Scopus
  54. C. P. Concepcion, C. Bonetti, and A. Ventura, “The microRNA-17-92 family of microRNA clusters in development and disease,” Cancer Journal, vol. 18, no. 3, pp. 262–267, 2012. View at Publisher · View at Google Scholar · View at Scopus
  55. D. Dakhlallah, K. Batte, Y. Wang et al., “Epigenetic regulation of miR-17 ~ 92 contributes to the pathogenesis of pulmonary fibrosis,” American Journal of Respiratory and Critical Care Medicine, vol. 187, pp. 397–405, 2013. View at Publisher · View at Google Scholar · View at Scopus
  56. T. Kodama, T. Takehara, H. Hikita et al., “Increases in p53 expression induce CTGF synthesis by mouse and human hepatocytes and result in liver fibrosis in mice,” The Journal of Clinical Investigation, vol. 121, pp. 3343–3356, 2011. View at Publisher · View at Google Scholar · View at Scopus
  57. H. Chen, C. Ren, C. Han, D. Wang, Y. Chen, and D. Fu, “Expression and prognostic value of miR-486-5p in patients with gastric adenocarcinoma,” PLoS One, vol. 10, no. 3, article e0119384, 2015. View at Publisher · View at Google Scholar · View at Scopus
  58. C. Ren, H. Chen, C. Han et al., “miR-486-5p expression pattern in esophageal squamous cell carcinoma, gastric cancer and its prognostic value,” Oncotarget, vol. 7, no. 13, pp. 15840–15853, 2016. View at Publisher · View at Google Scholar · View at Scopus
  59. G. Zhang, Z. Liu, G. Cui, X. Wang, and Z. Yang, “MicroRNA-486-5p targeting PIM-1 suppresses cell proliferation in breast cancer cells,” Tumor Biology, vol. 35, no. 11, pp. 11137–11145, 2014. View at Publisher · View at Google Scholar · View at Scopus
  60. J. Wang, X. Tian, R. Han et al., “Downregulation of miR-486-5p contributes to tumor progression and metastasis by targeting protumorigenic ARHGAP5 in lung cancer,” Oncogene, vol. 33, no. 9, pp. 1181–1189, 2014. View at Publisher · View at Google Scholar · View at Scopus
  61. X. Ji, B. Wu, J. Fan et al., “The anti-fibrotic effects and mechanisms of microRNA-486-5p in pulmonary fibrosis,” Scientific Reports, vol. 5, article 14131, 2015. View at Publisher · View at Google Scholar · View at Scopus
  62. J. E. Fish, M. M. Santoro, S. U. Morton et al., “miR-126 regulates angiogenic signaling and vascular integrity,” Developmental Cell, vol. 15, no. 2, pp. 272–284, 2008. View at Publisher · View at Google Scholar · View at Scopus
  63. S. Wang, A. B. Aurora, B. A. Johnson et al., “The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis,” Developmental Cell, vol. 15, no. 2, pp. 261–271, 2008. View at Publisher · View at Google Scholar · View at Scopus
  64. S. Nucera, A. Giustacchini, F. Boccalatte et al., “miRNA-126 orchestrates an oncogenic program in B cell precursor acute lymphoblastic leukemia,” Cancer Cell, vol. 29, no. 6, pp. 905–921, 2016. View at Publisher · View at Google Scholar · View at Scopus
  65. T. Otsubo, Y. Akiyama, Y. Hashimoto, S. Shimada, K. Goto, and Y. Yuasa, “MicroRNA-126 inhibits SOX2 expression and contributes to gastric carcinogenesis,” PLoS One, vol. 6, no. 1, article e16617, 2011. View at Publisher · View at Google Scholar · View at Scopus
  66. A. E. Frampton, J. Krell, J. Jacob, J. Stebbing, J. Castellano, and L. R. Jiao, “Loss of miR-126 is crucial to pancreatic cancer progression,” Expert Review of Anticancer Therapy, vol. 12, no. 7, pp. 881–884, 2012. View at Publisher · View at Google Scholar · View at Scopus
  67. X. Yang, B. B. Chen, M. H. Zhang, and X. R. Wang, “MicroRNA-126 inhibits the proliferation of lung cancer cell line A549,” Asian Pacific Journal of Tropical Medicine, vol. 8, no. 3, pp. 239–242, 2015. View at Publisher · View at Google Scholar · View at Scopus
  68. O. Slaby, M. Redova, A. Poprach et al., “Identification of microRNAs associated with early relapse after nephrectomy in renal cell carcinoma patients,” Genes, Chromosomes & Cancer, vol. 51, no. 7, pp. 707–716, 2012. View at Publisher · View at Google Scholar · View at Scopus
  69. X. Yang, H. Wu, and T. Ling, “Suppressive effect of microRNA-126 on oral squamous cell carcinoma in vitro,” Molecular Medicine Reports, vol. 10, no. 1, pp. 125–130, 2014. View at Publisher · View at Google Scholar · View at Scopus
  70. J. Meister and M. H. Schmidt, “miR-126 and miR-126*: new players in cancer,” The Scientific World Journal, vol. 10, pp. 2090–2100, 2010. View at Publisher · View at Google Scholar · View at Scopus
  71. T. Schepeler, A. Holm, P. Halvey et al., “Attenuation of the beta-catenin/TCF4 complex in colorectal cancer cells induces several growth-suppressive microRNAs that target cancer promoting genes,” Oncogene, vol. 31, no. 22, pp. 2750–2760, 2012. View at Publisher · View at Google Scholar · View at Scopus
  72. B. Liu, X. C. Peng, X. L. Zheng, J. Wang, and Y. W. Qin, “MiR-126 restoration down-regulate VEGF and inhibit the growth of lung cancer cell lines in vitro and in vivo,” Lung Cancer, vol. 66, no. 2, pp. 169–175, 2009. View at Publisher · View at Google Scholar · View at Scopus
  73. T. Sasahira, T. Kirita, U. K. Bhawall et al., “The expression of receptor for advanced glycation and products is associated with angiogenesis in human oral squamous cell carcinoma,” Virchows Archiv, vol. 450, no. 3, pp. 287–295, 2007. View at Publisher · View at Google Scholar · View at Scopus
  74. N. Zhu, D. Zhang, H. Xie et al., “Endothelial-specific intron-derived miR-126 is down-regulated in human breast cancer and targets both VEGFA and PIK3R2,” Molecular and Cellular Biochemistry, vol. 351, no. 1-2, pp. 157–164, 2011. View at Publisher · View at Google Scholar · View at Scopus
  75. T. Sasahira, M. Kurihara, U. K. Bhawal et al., “Downregulation of miR-126 induces angiogenesis and lymphangiogenesis by activation of VEGF-A in oral cancer,” British Journal of Cancer, vol. 107, no. 4, pp. 700–706, 2012. View at Publisher · View at Google Scholar · View at Scopus
  76. X. Zhu, H. Li, L. Long et al., “miR-126 enhances the sensitivity of non-small cell lung cancer cells to anticancer agents by targeting vascular endothelial growth factor a,” Acta Biochimica et Biophysica Sinica (Shanghai), vol. 44, no. 6, pp. 519–526, 2012. View at Publisher · View at Google Scholar · View at Scopus
  77. W. L. Wu, W. Y. Wang, W. Q. Yao, and G. D. Li, “Suppressive effects of microRNA-16 on the proliferation, invasion and metastasis of hepatocellular carcinoma cells,” International Journal of Molecular Medicine, vol. 36, no. 6, pp. 1713–1719, 2015. View at Publisher · View at Google Scholar · View at Scopus
  78. Y. Gu, X. D. Wang, J. J. Lu, Y. Y. Lei, J. Y. Zou, and H. H. Luo, “Effect of mir-16 on proliferation and apoptosis in human A549 lung adenocarcinoma cells,” International Journal of Clinical and Experimental Medicine, vol. 8, no. 3, pp. 3227–3233, 2015. View at Google Scholar
  79. R. W. Chen, L. T. Bemis, C. M. Amato et al., “Truncation in CCND1 mRNA alters miR-16-1 regulation in mantle cell lymphoma,” Blood, vol. 112, no. 3, pp. 822–829, 2008. View at Publisher · View at Google Scholar · View at Scopus
  80. W. Li, Z. Qi, Z. Wei et al., “Paeoniflorin inhibits proliferation and induces apoptosis of human glioma cells via microRNA-16 upregulation and matrix metalloproteinase-9 downregulation,” Molecular Medicine Reports, vol. 12, no. 2, pp. 2735–2740, 2015. View at Google Scholar
  81. G. Reid, M. E. Pel, M. B. Kirschner et al., “Restoring expression of miR-16: a novel approach to therapy for malignant pleural mesothelioma,” Annals of Oncology, vol. 24, no. 12, pp. 3128–3135, 2013. View at Publisher · View at Google Scholar · View at Scopus