EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

Figure 3

MMP9 presence and protein-level in AV-binding EVs from ascites. (a) Representative image of a zymogram to determine the presence of MMP9 in ascites samples from four individuals, where 1 μl of ascites (lanes 1, 3, 5, and 7) and AV-binding EVs from 500 μl ascites (lanes 2, 4, 6, and 8) were loaded. The sample in lanes 1 and 2 is from a patient with carcinosarcoma, the samples in lanes 3, 4, and 5, 6 are each from patients with HGSOC, and the sample in lanes 7 and 8 is from a patient with cirrhosis. (b) Box plot showing the difference in MMP9 band intensity analyzed by zymography in AV-EVs isolated from different ascites samples. (c) Correlation graph of MMP9-levels measured with zymography (y-axis) and ELISA (x-axis). (d) Box plot showing the difference in MMP9 amount analyzed by ELISA in AV-EVs isolated from different ascites samples (HGSOC = high-grade serous ovarian cancer).

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