Research Article

EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

Figure 4

Cryo-EM images of individual EVs from the ascites of patients with HGSOC (a), carcinosarcoma (b), or liver cirrhosis (c). The samples were labeled with AV-conjugated gold nanoparticles (10 nm diameter in (a) and (b), 4 nm diameter in (c)). In (a), a non-AV-binding EV (white arrow) is seen, next to an AV-labeled EV. The lipid bilayer surrounding EVs is clearly resolved by cryo-EM (black arrow in (b)). The white asterisks point to areas of the carbon support film. Scale bars: 100 nm in (a) and 50 nm in (b) and (c).
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