The Relationship between TP53 Gene Status and Carboxylesterase 2 Expression in Human Colorectal Cancer

Figure 1

The expression of p21 (a), Noxa (b), and CES2 (c) was quantified by real-time reverse transcriptase PCR. Human colorectal cancer cell lines with wild-type p53 (HCT116, HCT C, LS174T, LoVo, and RKO) and p53-null Caco-2 cells were treated with 10 μM nutlin-3a for 24 hours. For LoVo cells, 18S rRNA was used as the reference gene. For other cell types, GAPDH was used as the reference gene. Data represent the mean values ± SEM (–4). ; ; n.s.: no significance. A paired two-tailed t-test was used.

(a)

(b)

(c)